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SARS Spike RBD Protein

Product Info
Source

Recombinant SARS Spike RBD Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Arg306-Phe527.[Accession | P59594]

Molecular Weight

The protein has a predicted MW of 27.9 kDa. Due to glycosylation, the protein migrates to 36-46 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by SEC-HPLC

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

SARS Spike RBD on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of SARS Spike RBD is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized SARS Spike RBD at 0.5μg/ml (100μl/Well). Dose response curve for Human ACE2, hFc Tag with the EC50 of 83.8ng/ml determined by ELISA.

Background

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Synonyms

S protein,Spike glycoprotein,S glycoprotein

References

(1)Belouzard S , Chu V C , Whittaker G R . Activation Of The Sars Coronavirus Spike Protein Via Sequential Proteolytic Cleavage At Two Distinct Sites[J]. Proceedings of the National Academy of Sciences of the United States of America, 2009, 106(14):5871-5876.

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