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Human Siglec-10 Protein

Product Info
Source

Recombinant Human Siglec-10 Protein is expressed from Expi293 with His tag at the C-terminal. It contains Met17-Thr546.[Accession | Q96LC7]

Molecular Weight

The protein has a predicted MW of 59.2 kDa. Due to glycosylation, the protein migrates to 70-79 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by SEC-HPLC

Formulation

Lyophilized from 0.22μm filtered solution in 20mM PB, 150mM Nal, 25mM Arginine(pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Human Siglec-10 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human Siglec-10 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human Siglec-10 at 0.5μg/ml (100μl/Well). Dose response curve for Anti-Siglec-10 Ab., hFc Tag with the EC50 of 7.4ng/ml determined by ELISA.

Batch to Batch Consistency

Immobilized Human Siglec-10 at 0.5μg/ml (100μl/Well). Dose response curve for Anti-Siglec-10 Ab., hFc Tag with the EC50 of 7.3ng/ml / 7.7ng/ml / 7.4ng/ml determined by ELISA.

SPR Data

Human Siglec-10, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human CD52, mFc Tag with an affinity constant of 15.9μM as determined in SPR assay (Biacore T200).

Background

Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system.

Synonyms

siglec 10; siglec-10; siglec10; Sialic acid-binding Ig-like lectin 10; Siglec-like protein 2; SLG2

References

(1)Barkal AA, et al.CD24 signalling through macrophage Siglec-10 is a target for cancer immunotherapy.Nature. 2019 Jul 31. doi: 10.1038/s41586-019-1456-0.

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