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Human TIGIT Protein

Product Info
Source

Recombinant Human TIGIT Protein is expressed from Expi293 with His tag at the C-terminal. It contains Met22-Pro141.[Accession | Q495A1]

Molecular Weight

The protein has a predicted MW of 14 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Human TIGIT on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human TIGIT is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human TIGIT at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-TIGIT Antibody, hFc Tag with the EC50 of 49.1ng/ml determined by ELISA.

Blocking Data

Serial dilutions of Anti-TIGIT Antibody were added into Human TIGIT, His Tag : Biotinylated CD155, hFc Tag binding reactioins. The half maximal inhibitiory concentration(IC50) is 0.46μg/ml.

SPR Data

Human CD155, hFc Tag captured on Protein A chip, can bind Human TIGIT, His Tag with an affinity constant of 0.17μM as determined in a SPR assay(Biacore T200).

Background

TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.IGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.

Synonyms

TIGIT; VSIG9; VSTM3; WUCAM;T cell immunoreceptor with Ig and ITIM domains

References

(1)Yu X , Harden K , C Gonzalez L , et al. The surface protein TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells[J]. NATURE IMMUNOLOGY, 2008, 10(1):48-57.

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