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Human VEGF R2/KDR Protein

Product Info
Source

Recombinant Human VEGF R2/KDR Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Ala20-Glu764.[Accession | P35968]

Molecular Weight

The protein has a predicted MW of 86.2 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by SEC-HPLC

Formulation

Lyophilized from 0.22μm filtered solution in 20mM PB, 150mM NaCl (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Human VEGF R2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human VEGF R2 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human VEGF R2 at 0.5μg/ml (100μl/Well). Dose response curve for Anti-VEGF R2 Antibody, hFc Tag with the EC50 of 23.1ng/ml determined by ELISA.

Batch to Batch Consistency

Immobilized Human VEGF R2 at 0.5μg/ml (100μl/Well). Dose response curve for Anti-VEGF R2 Antibody, hFc Tag with the EC50 of 20.3/23.1/18.4ng/ml determined by ELISA.

Background

Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. The tyrosine kinase receptor vascular endothelial growth factor receptor 2 (VEGFR2) is a key regulator of angiogenesis.

Synonyms

CD309 antigen; CD309; EC 2.7.10; EC 2.7.10.1; Fetal liver kinase 1; fetal liver kinase-1; Flk1; Flk-1; FLK1tyrosine kinase growth factor receptor; KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; vascular endothelial growth factor receptor 2; VEGF R2; VEGFR; VEGFR2; VEGFR-21

References

(1)Miettinen M , Rikala M S , Rys J , et al. Vascular Endothelial Growth Factor Receptor 2 as a Marker for Malignant Vascular Tumors and Mesothelioma:, An Immunohistochemical Study of 262 Vascular Endothelial and 1640 Nonvascular Tumors[J]. The American Journal of Surgical Pathology, 2012, 36(4):629-39.

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