The best CD3 protein on the market

T cell receptor (TCR)‐based immunotherapy one of the top topics in drug discovery. As cluster of differentiation 3 (CD3) proteins play a key role in T-cell development and TCR signal transduction, researchers have been interested in CD3-associated antibodies recently.
CD3 is one kind of transmembrane protein containing four subunits: CD3D, CD3E, CD3G and CD3Z. Heterodimer composed either of the CD3D&CD3E or CD3E&CD3G can bind the TCR alpha/beta chains to form a TCR/CD3 complex on the surface of T-cells for antigen recognition (Fig. 1). Therefore, CD3 becomes the most popular targets in bispecific antibody discovery (Typically, one binding to CD3 on T cells and another against a tumor associated antigen).
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Fig. 1 TCR/CD3 complex for antigen recognition of T cells.
Based on our featured SAMSTM platform, Kactus has developed a series of CD3 proteins, especially heterodimers CD3D&CD3E and CD3E&CD3G. Two subunits were designed to be homogeneously expressed and composed as heterodimer but not homodimer, avoiding the potential loss of bioactivity or batch-to-batch inconsistency. Further we tested the bioactivity of heterodimers using multiple reported antibodies.
Advantages
Homogeneously expressed subunits;
High bioactivity and high purity (>95%);
High degree of batch-to-batch consistency;
Structure-based protein expression system for native antigen mimic;
Successfully used for bispecific antibody research, showed high affinity with more antibodies*.
* Based on our customers' feedback.
Our Data
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Fig. 2 Human CD3D&CD3E and CD3E&CD3G heterodimers. Subunits homogeneously expressed verified by Tris-Bis PAGE, purity determined by HPLC and bioactivity assay by ELISA.
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Fig. 3 Cynomolgus CD3D&CD3E and CD3E&CD3G heterodimers. Subunits homogeneously expressed verified by Tris-Bis PAGE, purity determined by HPLC and bioactivity assay by ELISA.
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Fig. 4 Batch-to-batch consistency.
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Fig. 5 Stability testing in an accelerated condition (37°C).
Product List
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Cat. No. Protein Name Species Tag Exp. System
CDD-HM101 Human CD3 Delta Protein Human C-His Expi293 Inquiry Now
CDE-HM101 Human CD3 Epsilon Protein Human C-His Expi293 Inquiry Now
CDE-CM101 Cynomolgus CD3E/CD3 Epsilon Protein Cynomolgus C-His Expi293 Inquiry Now
CDE-CM201 Cynomolgus CD3E/CD3 Epsilon Protein Cynomolgus C-hFc Expi293 Inquiry Now
CD3-CM201 Cynomolgus CD3E&CD3D/CD3 Epsilon&CD3 Delta Protein Cynomolgus C-hFc Expi293 Inquiry Now
CD3-CM202 Cynomolgus CD3E&CD3G/CD3 Epsilon&CD3 Gamma Protein Cynomolgus C-hFc Expi293 Inquiry Now
CD3-HM105 Human CD3E&CD3D/CD3 Epsilon&CD3 Delta Protein Human C-His Expi293 Inquiry Now
CD3-HM205 Human CD3E&CD3D/CD3 Epsilon&CD3 Delta Protein Human C-hFc Expi293 Inquiry Now
CD3-MM205 Mouse CD3E&CD3D/CD3 Epsilon&CD3 Delta Protein Mouse C-hFc Expi293 Inquiry Now
CD3-HM257 Human CD3E&CD3G/CD3 Epsilon&CD3 Gamma Protein Human C-hFc Expi293 Inquiry Now
CD3-HM557B Biotinylated Human CD3E&CD3G/CD3 Epsilon&CD3 Gamma Protein Human C-hFc-Avi Expi293 Inquiry Now
CD3-HM505B Biotinylated Human CD3E&CD3D/CD3 Epsilon&CD3 Delta Protein Human C-hFc-Avi Expi293 Inquiry Now
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