LILRBs, a new target for first-in-class drug discovery

MHC class I (MHC-I) polymorphisms are associated with the outcome of some viral infections and autoimmune diseases. MHC-I proteins present antigenic peptides and are recognized by receptors, thus enabling the immune system to detect self-antigens and eliminate targets lacking self or expressing foreign antigens.
Leukocyte Ig-like receptor (LILR) family is one kind of these receptors that either activate (LILRA members) or suppress (LILRB members) immune cell functions. These Type 1 transmembrane glycoproteins are composed of two or four extracellular Ig-like domains that bind ligands. As LILRBs have a similar immunoreceptor tyrosine-based inhibitory motif (ITIM) like immune checkpoint proteins such as CTLA4 and PD-1 (Fig. 1), they became hot targets for drug development, especially used for discovery of first-in-class drugs. Multiple clinical/preclinical studies showed the potential broad anti-tumor effects of LILR family proteins, such as BND-22 (Biond), IO-202 (Immune Onc), JTX-8064 (Jounce Therapeutics) and NC410 (NextCure).
1.png
Fig. 1 LILRBs binding ligands.
Based on our featured SAMSTM platform, Kactus has developed a series of proteins/domains in LILR family. As LILRB1 and LILRB2 preferentially binds to HLA-G for MHC-I binding, we also developed multiple forms of HLA-G complex for further applications.
Advantages
Low-endotoxin (<1EU per μg by the LAL method) and high-purity (>95%);
High avidity;
High degree of batch-to-batch consistency;
Offering biotinylated proteins, with highest bioactivity and sensitivity.
Our Data
2.png
3.png
Fig. 2 Human LILRB2/LILRB2 domain 1&2. Bioactivity and inhibition assay by ELISA.
4.png
Fig. 3 Human LILRB2 D1&D2 captured on Protein A Chip can bind HLA-G, His Tag with an affinity constant of 6.5 μM as determined in SPR assay (Biacore T200).
5.png
Fig. 4 Human LILRB2 captured on Protein A Chip can bind HLA-G, His Tag with an affinity constant of 9.20 nM as determined in SPR assay (Biacore T200).
Product List
Contact us today for a free sample
Cat. No. Protein Name Species Tag Exp. System
LIL-HM4A1 Human LILRA1/CD85i/LIR-6 Protein Human C-His-Avi Expi293 Inquiry Now
LIL-HM4A2 Human LILRA2/CD85h/ILT1 Protein Human C-His-Avi Expi293 Inquiry Now
LIL-HM4A3 Human LILRA3/CD85e Protein Human C-His-Avi Expi293 Inquiry Now
LIL-HM4A4 Human LILRA4/CD85g Protein Human C-His-Avi Expi293 Inquiry Now
LIL-CM1A4 Cynomolgus LILRA4/CD85g Protein Cynomolgus C-His Expi293 Inquiry Now
LIL-HM4A5 Human LILRA5/CD85f/ILT11 Protein Human C-His-Avi Expi293 Inquiry Now
LIL-HM4A6 Human LILRA6/CD85b/ILT8 Protein Human C-His-Avi Expi293 Inquiry Now
LIL-HM4B1 Human LILRB1/CD85j/ILT2 Protein Human C-His-Avi Expi293 Inquiry Now
LIL-HM2B1 Human LILRB1/CD85j/ILT2 Protein Human C-hFc Expi293 Inquiry Now
LIL-CM1B1 Rhesus macaque LILRB1/CD85j/ILT2 Protein Rhesus macaque C-His Expi293 Inquiry Now
LIL-HM11D Human LILRB1/CD85j/ILT2 domain1&2 Protein Human C-His Expi293 Inquiry Now
LIL-HM4B2 Human LILRB2/CD85d/ILT4 Protein Human C-His-Avi Expi293 Inquiry Now
LIL-HM2B2 Human LILRB2/CD85d/ILT4 Protein Human C-hFc Expi293 Inquiry Now
LIL-HM12D Human LILRB2/CD85d/ILT4 domain1&2 Protein Human C-His Expi293 Inquiry Now
LIL-CM1B2 Cynomolgus LILRB2/CD85d/ILT4 Protein Cynomolgus C-His Expi293 Inquiry Now
LIL-HM4B3 Human LILRB3/CD85a/ILT5 Protein Human C-His-Avi Expi293 Inquiry Now
LIL-HM4B4 Human LILRB4/CD85k/ILT3 Protein Human C-His-Avi Expi293 Inquiry Now
LIL-HM2B4 Human LILRB4/CD85k/ILT3 Protein Human C-hFc Expi293 Inquiry Now
LIL-MM1B4 Mouse LILRB4/CD85k/ILT3 Protein Mouse C-His Expi293 Inquiry Now
LIL-CM1B4 Cynomolgus LILRB4/CD85k/ILT3 Protein Cynomolgus C-His Expi293 Inquiry Now
LIL-HM4B5 Human LILRB5/CD85c/LIR-8 Protein Human C-His-Avi Expi293 Inquiry Now
ANG-HM4L2 Human ANGPTL2 Protein Human C-His-Avi Expi293 Inquiry Now
ANG-HM403 Human Angiopoietin-like 3/ANGPTL3 Protein Human C-His-Avi Expi293 Inquiry Now
ANG-HM1L4 Human Angiopoietin-like 4/ANGPTL4 Protein Human N-His Expi293 Inquiry Now
APO-HM101 Human Apolipoprotein E/APOE Protein Human N-His Expi293 Inquiry Now
HLG-HE41F Human HLA-G Free Heavy Chain Protein Human C-His-Avi E.coli Inquiry Now
HLG-HM41C Human HLA-G Complex Protein Human C-His-Avi Expi293 Inquiry Now
HLG-HM41CT Human HLA-G Complex Tetramer Protein Human C-His-Avi Expi293 Inquiry Now
Contact us today for a free sample
More products from our catalog
Kactus Biosystems Co.Ltd
Online Message