SAMSTM_Mam MHCTM

T lymphocytes, key mediators of the adaptive immune response, are activated when their T-cell receptors (TCRs) interact with cognate antigenic peptides displayed by major histocompatibility complex molecules (MHC). E. coli expression and in vitro protein refolding is a common method to produce MHC-I complex. Based on SAMSTM_Mam MHCTM platform, KACTUS succeeded in expressing biotinylated MHC-I complex in mammalian cell Expi293. The resulting MHC-I complexes show high affinities with benchmark antibodies and have low endotoxin levels – ideal for endotoxin sensitive cell-based assay. KACTUS also provide MHC tetramer complex which has greater avidity to the T cell mand maintains more stable binding.
1.png
Fig. 1 MHC class I maturation, ligand binding, and presentation on the cell surface
Advantages
Mammalian expression with Post-translational modification;
Good binding affinity;
Low endotoxin;
Flexible choice: monomer or tetramer.
Our Data
2.jpg
Fig. 2 Bioactivity assay of Kactus human HLA-A*0201 WT-1 peptide complex tetramer protein by ELISA.
3.png
ka (1/Ms) kd (1/Ms) KD (M)
1.60E 06 3.00E-04 1.79E-10
Fig. 3 SPR result of Kactus human HLA-A*0201 WT-1 peptide complex protein tetramer with WT-1 antibody.
Product List
Cat. No. Protein Name Peptide Species Tag Exp. System
MHC-HM402 Human gp100 (HLA-A*02:01) Complex Protein YLEPGPVTA Human C-His-Avi Expi293 Inquiry Now
MHC-HM402B Biotinylated Human gp100 (HLA-A*02:01) Complex Protein YLEPGPVTA Human C-His-Avi Expi293 Inquiry Now
MHC-HM401 Human MAGE-A4 (HLA-A*02:01) Complex Protein GVYDGREHTV Human C-His-Avi Expi293 Inquiry Now
MHC-HM401B Biotinylated Human MAGE-A4 (HLA-A*02:01) Complex Protein GVYDGREHTV Human C-His-Avi Expi293 Inquiry Now
MHC-HM405B Biotinylated Human NY-ESO-1(HLA-A*02:01) Complex Protein SLLMWITQC Human C-His-Avi Expi293 Inquiry Now
MHC-HM405T Human NY-ESO-1(HLA-A*02:01) Complex Tetramer Protein SLLMWITQC Human C-His-Avi Expi293 Inquiry Now
MHC-HM431 Human WT-1 (HLA-A*02:01) Complex Protein RMFPNAPYL Human C-His-Avi Expi293 Inquiry Now
MHC-HM431B Biotinylated Human WT-1 (HLA-A*02:01) Complex Protein RMFPNAPYL Human C-His-Avi Expi293 Inquiry Now
MHC-HM431T Human WT-1 (HLA-A*02:01) Complex Tetramer Protein RMFPNAPYL Human C-His-Avi Expi293 Inquiry Now
MHC-HM406B Biotinylated Human HLA-E Complex Protein VMAPRTLVL Human C-His-Avi Expi293 Inquiry Now
MHC-HM406T Human HLA-E Complex Tetramer Protein VMAPRTLVL Human C-His-Avi Expi293 Inquiry Now
HLG-HE41F Human HLA-G Free Heavy Chain Protein / Human C-His-Avi E.coli Inquiry Now
HLG-HM41C Human HLA-G Complex Protein RIIPRHLQL Human C-His-Avi Expi293 Inquiry Now
HLG-HM41CB Biotinylated Human HLA-G Complex Protein RIIPRHLQL Human C-His-Avi Expi293 Inquiry Now
HLG-HM41CT Human HLA-G Complex Tetramer Protein RIIPRHLQL Human C-His-Avi Expi293 Inquiry Now
HLG-HM41CTB Biotinylated Human HLA-G Complex Tetramer Protein RIIPRHLQL Human C-His-Avi Expi293 Inquiry Now
More products from our catalog
Kactus Biosystems Co.Ltd
Online Message