Biotinylated Human CEACAM-5/CD66e Protein (CAM-HM405B)
Catalog: CAM-HM405B
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Product Description | Recombinant Biotinylated Human CEACAM-5/CD66e Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.; It contains Lys35-Ala685. |
Product Category | Recombinant Protein |
Target | CEACAM-5/CD66e |
Synonyms | CEACAM-5; CD66e; CEA; Meconium antigen 100; Carcinoembryonic; CEACD66e; DKFZp781M2392 |
Gene/Protein ID | Q8N4D0 |
Species | Human |
Exact Sequence | Lys35-Ala685 |
Molecular Weight | The protein has a predicted MW of 74.2 kDa. Due to glycosylation, the protein migrates to 115-145 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Anti-CEACAM-5 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CEACAM-5, His Tag with the EC50 of 41.1ng/ml determined by ELISA. See testing image for detail. |
Product Tag | C-His-Avi |
Express System | Expi293 |
Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
Endotoxin | Less than 1EU per μg by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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