Biotinylated Human TNFSF15 Protein (FSF-HM415B)

  • Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Biotinylated: yes
  • Activity: Immobilized Mouse DR3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TNFSF15, His Tag with the EC50 of 26.0ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Exact Sequence: Leu72-Leu251

Catalog: FSF-HM415B

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Product Description Recombinant Biotinylated Human TNFSF15 Protein is expressed from HEK293 with His tag and Avi tag at the N-terminus.It contains Leu72-Leu251.

Product Background

TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2.

Product Category

Recombinant Protein

Target

TNFSF15

Gene/Protein ID

O95150-1

Species

Human

Exact Sequence

Leu72-Leu251
Molecular Weight The protein has a predicted MW of 23.38 kDa. Due to glycosylation, the protein migrates to 27-37 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Mouse DR3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TNFSF15, His Tag with the EC50 of 26.0ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

N-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Liquid
Shipping Shipped with dry ice.
Formulation Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Mouse DR3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TNFSF15, His Tag with the EC50 of 26.0ng/ml determined by ELISA (QC Test).
Immobilized Biotinylated Human TNFSF15, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA.
Biotinylated Human TNFSF15 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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