Biotinylated Human TNFSF15 Protein (FSF-HM415B)
|Recombinant Biotinylated Human TNFSF15 Protein is expressed from HEK293 with His tag and Avi tag at the N-terminus.It contains Leu72-Leu251.
|TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2.
|The protein has a predicted MW of 23.38 kDa. Due to glycosylation, the protein migrates to 27-37 kDa based on Tris-Bis PAGE result.
|Immobilized Mouse DR3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TNFSF15, His Tag with the EC50 of 26.0ng/ml determined by ELISA (QC Test). See testing image for detail.
|> 95% as determined by Tris-Bis PAGE
|Less than 1EU per ug by the LAL method.
|Shipped with dry ice.
|Supplied as 0.22 um filtered solution in PBS (pH 7.4).
|Stability And Storage
|Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.