Cynomolgus/Rhesus macaque CD19 Protein, Ultra Low Endotoxin (CD1-CM119-UL)
  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: The affinity constant of 0.46 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gln21-Trp291
Catalog #CD1-CM119-UL
Regular price $381.00
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Product Description

Recombinant Cynomolgus/Rhesus macaque CD19 Protein is expressed from HEK293 with His tag at the N-terminus; It contains Gln21-Trp291.

Product Background

CD19 is a 95 kDa transmembrane glycoprotein that plays a central role in B cell activation and humoral immune responses. Functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca2+ stores.

Product Category

Recombinant Protein / Antibody discovery / CAR-T targets

Protein

CD19

Synonyms

CD19; B4; CVID3; Leu-12; MGC12802

Accession

Species

Cynomolgus/Rhesus macaque

Biotinylated

no

Amino Acid Range

Gln21-Trp291

Molecular Weight

The protein has a predicted MW of 30.96 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.

Activity

The affinity constant of 0.46 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

N-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin

Less than 0.01 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Anti-CD19 Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus/Rhesus macaque CD19, His Tag with an affinity constant of 0.46 uM as determined in SPR assay (Biacore T200). Anti-CD19 Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus/Rhesus macaque CD19, His Tag with an affinity constant of 0.46 uM as determined in SPR assay (Biacore T200).
Cynomolgus/Rhesus macaque CD19 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus/Rhesus macaque CD19 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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support@kactusbio.us

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