DNase I (RNase-free) (DNI-EE001)
Catalog: DNI-EE001
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| Product Description | DNase I is an endonuclease that digests single- and double-stranded DNA to oligonucleotide containing a 5' phosphate and 3'-hydroxylated ends. Both single- and double-stranded DNA are degraded by DNaseI. It is an essential enzyme for the efficient digestion of DNA during RNA purification, suitable for all molecular diagnostics and in vitro mRNA synthesis applications. Expressed in an E.coli strain with DNaseI gene isolated from bovine pancreas. |
Product Category |
Enzyme |
| Application | In Vitro Synthesis(IVT) Removal of DNA from RNA samples DNase I footprinting |
| Concentration | 4U/UL |
| Unit Definition | One unit is defined as the amount of enzyme which will completely degrade 1μg of pBR322 DNA in 10 minutes at 37°C in DNase I Reaction Buffer. |
| Molecular Weight | 31KD |
Express System |
E.coli |
| Form | Liquid |
| Shipping | Shipped with blue ice. |
| Stability And Storage | -20°C for 12 months. Avoid repeated freeze-thaw cycles. |
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Figure 1. 1µg DNA was added to various activity units of DNase I in a total reaction volume of 10µL. Compared with competitors 1 and 2, DNase I of KACTUS has a stronger digestive effect. |
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Figure 2. Different concentrations of ions were added to the transcriptional reaction. Results show DNase I is inhibited by Na+ and K+. |
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