Mouse GPC3/Glypican 3 Protein (GPC-MM131)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse GPC3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln25-Met557

Catalog: GPC-MM131

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Product Description Recombinant Mouse GPC3/Glypican 3 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gln25-Met557.

Product Background

Glypican-3 is a protein ,which is encoded by the GPC3 gene in humans.The protein core of GPC3 consists of two subunits, where the N-terminal subunit has a size of ~40 kDa and the C-terminal subunit is ~30 kDa.Glypican 3 is a potential therapeutic target for treating liver cancer and other cancers. Several therapeutic anti-GPC3 antibodies have been developed.

Product Category

Recombinant Protein

Target

GPC3/Glypican 3

Gene/Protein ID

Q8CFZ4

Species

Mouse

Exact Sequence

Gln25-Met557
Molecular Weight The protein has a predicted MW of 61.62 kDa. Due to furin-like convertase cleavage site and glycosylation, the protein migrates to 32-42 kDa and 75-85 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Mouse GPC3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Liquid
Shipping Shipped with dry ice.
Formulation Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Mouse GPC3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA.
The purity of Mouse GPC3 is greater than 95% as determined by SEC-HPLC.
Mouse GPC3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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