Product Description |
HRV 3C Protease is a recombinant cysteine protease originated from the Human Rhinovirus 3C (HRV3C). It recognizes and cleavse the octapeptide sequence Leu-Glu-Val-Leu-Phe-Gln↓Gly-Pro (LEVLFQ↓GP) or the core pentapeptide sequence Leu-Phe-Gln-Gly-Pro (LFQ↓GP) at low temperatures (4°C). This cleavage is used to remove tags from fusion proteins that contain the HRV 3C Protease recognition sequence, thereby obtaining specific protein fragments. |
Applications |
Tag removal of fusion proteins |
Concentration |
10U/ul |
Unit Definition |
One active unit is defined as, In a 1x HRV 3C Buffer (50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, pH 7.0), the amount of enzyme required to achieve more than 95% cleavage efficiency for 100 µg of substrate over 16 hours at 4°C. |
Product Tag |
The product contains a His-tag and can be removed after cleavage using Ni-NTA resin. |
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