Name: Human B7-H4 Protein, Ultra Low Endotoxin (BH7-HM174-UL)
Brand: KactusBio
SKU: BH7-HM174-UL-100UG
Price: 236.0 USD
Availability: InStock

Human B7-H4 Protein, Ultra Low Endotoxin (BH7-HM174-UL)

Immobilized Human B7-H4, His Tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-B7-H4 Antibody, hFc Tag with the EC50 of 6.7 ng/ml determined by ELISA.

Human B7-H4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Loaded Anti-B7-H4 Antibody, hFc Tag on ProA-Biosensor can bind Human B7-H4, His-Avi Tag with an affinity constant of 8.19 nM as determined in BLI assay (Gator® Prime).

The purity of Human B7-H4 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human B7-H4, His Tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-B7-H4 Antibody, hFc Tag with the EC50 of 6.7 ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Phe29-Ala258

Catalog #BH7-HM174-UL

Size

100UG

500UG

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Product Description	Recombinant Human B7-H4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe29-Ala258.
Product Background	B7-H4, also known as B7x and B7S1, is a 50-80 kDa glycosylated member of the B7 family of immunomodulatory proteins. B7-H4 is up-regulated in several carcinomas in correlation with tumor progression and metastasis. A soluble form of B7-H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, also in correlation with advanced disease status-.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	B7-H4
Synonyms	B7H4; B7-H4; B7S1; B7h.5; B7x; FLJ22418; VTCN1; PRO1291
Accession	Q7Z7D3-1
Species	Human
Biotinylated	no
Amino Acid Range	Phe29-Ala258
Molecular Weight	The protein has a predicted MW of 28.2 kDa. Due to glycosylation, the protein migrates to 52-68 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human B7-H4, His Tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-B7-H4 Antibody, hFc Tag with the EC50 of 6.7 ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.01 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human B7-H4, His Tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-B7-H4 Antibody, hFc Tag with the EC50 of 6.7 ng/ml determined by ELISA.
	Human B7-H4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Anti-B7-H4 Antibody, hFc Tag on ProA-Biosensor can bind Human B7-H4, His-Avi Tag with an affinity constant of 8.19 nM as determined in BLI assay (Gator® Prime).
	The purity of Human B7-H4 is greater than 95% as determined by SEC-HPLC.