Human CD8 alpha/CD8A Protein (CD8-HM48A)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Human CD8 alpha, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD8 Antibody, hFc Tag with the EC50 of 26.3ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ser22-Asp182

Catalog: CD8-HM48A

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Product Description Recombinant Human CD8 alpha/CD8A Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.; It contains Ser22-Asp182.
Product Category Recombinant Protein
Target CD8 alpha/CD8A
Synonyms CD8a; Lyt-2; CD8; Leu2; MAL; p32; CD8a molecule; OKT8 T-cell antigen
Gene/Protein ID P01732-1
Species Human
Exact Sequence Ser22-Asp182
Molecular Weight The protein has a predicted MW of 20.5 kDa. Due to glycosylation, the protein migrates to 31-35 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human CD8 alpha, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD8 Antibody, hFc Tag with the EC50 of 26.3ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CD8 alpha, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD8 Antibody, hFc Tag with the EC50 of 26.3ng/ml determined by ELISA.
The purity of Human CD8 alpha/CD8A is greater than 95% as determined by SEC-HPLC.
Human CD8 alpha/CD8A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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