Human CEACAM-5/CD66e Protein (CAM-HM405)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CEACAM-5 at 0.5μg/ml (100μl/well). Dose response curve for Anti-CEACAM Antibody, hFc Tag with the EC50 of 14.8ng/ml determined by ELISA. The affinity constant of 0.12nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Lys35-Ala685

Catalog: CAM-HM405

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Product Description Recombinant Human CEACAM-5/CD66e Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Lys35-Ala685.
Product Category Recombinant Protein
Target CEACAM-5/CD66e
Synonyms CEACAM-5; CD66e; CEA; Meconium antigen 100; Carcinoembryonic; CEACD66e; DKFZp781M2392
Gene/Protein ID Q8N4D0
Species Human
Exact Sequence Lys35-Ala685
Molecular Weight The protein has a predicted MW of 74.2 kDa. Due to glycosylation, the protein migrates to 115-150 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human CEACAM-5 at 0.5μg/ml (100μl/well). Dose response curve for Anti-CEACAM Antibody, hFc Tag with the EC50 of 14.8ng/ml determined by ELISA. The affinity constant of 0.12nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human CEACAM-5, His Tag captured on CM5 Chip via Anti-His Antibody can bind Anti-CEACAM5 Antibody, hFc Tag with an affinity constant of 0.12nM as determined in SPR assay (Biacore T200).
Immobilized Human CEACAM-5 at 0.5μg/ml (100μl/well). Dose response curve for Anti-CEACAM Antibody, hFc Tag with the EC50 of 14.8ng/ml determined by ELISA.
The purity of Human CEACAM-5 is greater than 95% as determined by SEC-HPLC.
Human CEACAM-5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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