Human DLL4 Protein (DLL-HM404)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human DLL4, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-DLL4 Antibody, hFc Tag with the EC50 of 49.2ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ser27-Pro524

Catalog: DLL-HM404

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Product Description Recombinant Human DLL4 Protein is expressed from Expi293 with His tag at the C-terminal.It contains Ser27-Pro524.
Product Category Recombinant Protein
Target DLL4
Synonyms DLL4; MGC126344; notch ligand DLL4; Delta4; Delta-like 4
Gene/Protein ID Q9NR61
Species Human
Exact Sequence Ser27-Pro524
Molecular Weight The protein has a predicted MW of 55.7 kDa. Due to glycosylation, the protein migrates to 62-68 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human DLL4, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-DLL4 Antibody, hFc Tag with the EC50 of 49.2ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human DLL4, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-DLL4 Antibody, hFc Tag with the EC50 of 49.2ng/ml determined by ELISA.
The purity of Human DLL4 is greater than 95% as determined by SEC-HPLC.
Human DLL4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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