Human DR3/TNFRSF25 Protein (DR3-HM201)

  • Express System: Expi293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human DR3, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-DR3 Antibody, hFc Tag with the EC50 of 0.20μg/ml determined by ELISA (QC Test). The affinity constant of 13.65 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gln25-Gln199

Catalog: DR3-HM201

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Product Description Recombinant Human DR3/TNFRSF25 Protein is expressed from Expi293 with hFc tag at the C-terminal.It contains Gln25-Gln199.
Product Category Recombinant Protein
Target DR3/TNFRSF25
Synonyms APO3; DDR3; DR3; TNFRSF12; WSL; WSL1; TNFRSF25; LARD; TR3; TRAMP; WSL-LR
Gene/Protein ID AAI17190
Species Human
Exact Sequence Gln25-Gln199
Molecular Weight The protein has a predicted MW of 45.6 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human DR3, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-DR3 Antibody, hFc Tag with the EC50 of 0.20μg/ml determined by ELISA (QC Test). The affinity constant of 13.65 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human DR3, hFc Tag immobilized on CM5 Chip can bind Anti-DR3 Antibody with an affinity constant of 13.65 nM as determined in SPR assay (Biacore T200).
Immobilized Human DR3, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-DR3 Antibody, hFc Tag with the EC50 of 0.20μg/ml determined by ELISA (QC Test).
The purity of Human DR3 is greater than 95% as determined by SEC-HPLC.
Human DR3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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