Name: Human DR3/TNFRSF25 Protein, Ultra Low Endotoxin (DR3-HM603-UL)
Brand: KactusBio
SKU: DR3-HM603-UL-100UG
Price: 327.0 USD
Availability: InStock

Human DR3/TNFRSF25 Protein, Ultra Low Endotoxin (DR3-HM603-UL)

Human DR3, monomeric Fc Tag captured on CM5 Chip via Protein A can bind Human TNFSF15 Trimer, His Tag with an affinity constant of 0.10 nM as determined in SPR assay (Biacore T200).

Immobilized Human TNFSF15 Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human DR3, monomeric Fc Tag with the EC50 of 2.2ug/ml determined by ELISA (QC Test).

Human DR3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Express System: HEK293
Product Tag: C-His-monomeric Fc
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human TNFSF15 Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human DR3, monomeric Fc Tag with the EC50 of 2.2ug/ml determined by ELISA (QC Test). The affinity constant of 0.10 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gln25-Gln199

Catalog #DR3-HM603-UL

Size

100UG

500UG

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Product Description	Recombinant Human DR3/TNFRSF25 Protein is expressed from HEK293 with His tag and monomeric Fc tag at the C-terminus.It contains Gln25-Gln199.
Product Background	Death Receptor 3 (DR3), also known as TNFRSF25, TRAMP, LARD, or WSL-1, is a death-domain-containing TNF-family receptor that, like its closest paralog TNFR1, binds the adaptor molecule TRADD through its cytoplasmic death domain. TRADD recruitment endows DR3 with dual-signaling capability to activate NF−κB and MAP-kinase signaling or alternatively trigger caspase activation and programmed cell death.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	DR3/TNFRSF25
Synonyms	APO3; DDR3; DR3; TNFRSF12; WSL; WSL1; TNFRSF25; LARD; TR3; TRAMP; WSL-LR
Accession	AAI17190
Species	Human
Biotinylated	no
Amino Acid Range	Gln25-Gln199
Molecular Weight	The protein has a predicted MW of 49.80 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human TNFSF15 Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human DR3, monomeric Fc Tag with the EC50 of 2.2ug/ml determined by ELISA (QC Test). The affinity constant of 0.10 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-monomeric Fc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 0.01 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human DR3, monomeric Fc Tag captured on CM5 Chip via Protein A can bind Human TNFSF15 Trimer, His Tag with an affinity constant of 0.10 nM as determined in SPR assay (Biacore T200).
	Immobilized Human TNFSF15 Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human DR3, monomeric Fc Tag with the EC50 of 2.2ug/ml determined by ELISA (QC Test).
	Human DR3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.