Human GDF15 Protein (GDF-HM315)
Catalog: GDF-HM315
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Product Description | Recombinant Human GDF15 Protein is expressed from Expi293 with mFc (IgG2a) tag at the N-terminal.It contains Ala197-Ile308. |
Product Category | Recombinant Protein |
Target | GDF15 |
Gene/Protein ID | Q99988 |
Species | Human |
Exact Sequence | Ala197-Ile308 |
Molecular Weight | The protein has a predicted MW of 37.46 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Human GFRAL, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human GDF15, mouse IgG2a Fc Tag with the EC50 of 8.0ng/ml determined by ELISA. See testing image for detail. |
Product Tag | N-mFc |
Express System | Expi293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per μg by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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