Human IL-1 Rrp2/IL-1 R6 Protein, Ultra Low Endotoxin (IL1-HM4L2-UL)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-1 Rrp2, His Tag at 1ug/ml (100ul/Well). Dose response curve for Anti-IL-1Rrp2 Antibody, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Asp20-Arg335
Catalog #IL1-HM4L2-UL
Regular price $327.00
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Product Description

Recombinant Human IL-1 Rrp2/IL-1 R6 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp20-Arg335.

Product Background

The Interleukin 1 receptor family (IL-1 R) comprises at least eleven members including IL-1 RI (IL-1 R1), IL-1 RII (IL-1 R2), IL-1 RAcP (IL‑1 R3), ST2 (T1/IL-1 R4), IL-18 Ra (IL-1 Rrp/IL-1 R5), IL-1 Rrp2 (IL-1 RL2/IL-1 R6), IL-18 Rb (AcPL/IL-1 R7), IL-1RAPL‑1 (TIGIRR‑2/IL‑1 R8), and TIGIRR-1 (IL-1 R9). IL1RL2 is a receptor for interleukin-36 (IL36A, IL36B and IL36G). After binding to interleukin-36 associates with the coreceptor IL1RAP to form the interleukin-36 receptor complex which mediates interleukin-36-dependent activation of NF-kappa-B, MAPK and other pathways.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

IL-1 Rrp2/IL-1 R6

Synonyms

IL-1 R6; IL-1 Rrp2; IL1RL2; IL1Rrp2; IL1RRP2; IL-36R

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Asp20-Arg335

Molecular Weight

The protein has a predicted MW of 39.1 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human IL-1 Rrp2, His Tag at 1ug/ml (100ul/Well). Dose response curve for Anti-IL-1Rrp2 Antibody, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 0.01 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human IL-1 Rrp2, His Tag at 1ug/ml (100ul/Well). Dose response curve for Anti-IL-1Rrp2 Antibody, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA. Immobilized Human IL-1 Rrp2, His Tag at 1ug/ml (100ul/Well). Dose response curve for Anti-IL-1Rrp2 Antibody, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA.
Human IL-1 Rrp2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IL-1 Rrp2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-1 Rrp2 is greater than 95% as determined by SEC-HPLC. The purity of Human IL-1 Rrp2 is greater than 95% as determined by SEC-HPLC.

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