Human PVRIG Protein (PVR-HM301)
Catalog: PVR-HM301
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| Product Description | Recombinant Human PVRIG Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Thr41-Asp171. |
| Product Category | Recombinant Protein |
| Target | PVRIG |
| Synonyms | C7orf15; CD112R; MGC104322; MGC138297; MGC2463; PVRIG; ALS2CR18; ALS2CR9; LPD; PREL-2; PREL2; RalGDS/AF-6; RMO1 |
| Gene/Protein ID | Q6DKI7-1 |
| Species | Human |
| Exact Sequence | Thr41-Asp171 |
| Molecular Weight | The protein has a predicted MW of 39.6 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human Nectin-2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 0.22ug/ml determined by ELISA (QC Test). The affinity constant of 0.349 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-mFc |
| Express System | HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human PVRIG, mFc Tag captured on CM5 Chip via Anti-Mouse Antibody can bind Human Nectin-2, His Tag with an affinity constant of 0.349 uM as determined in SPR assay (Biacore T200). |
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Immobilized Human Nectin-2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 0.22ug/ml determined by ELISA (QC Test). |
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Immobilized Human PVRIG, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-PVRIG Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA. |
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The purity of Human PVRIG is greater than 95% as determined by SEC-HPLC. |
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Human PVRIG on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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