Human CA9/Carbonic Anhydrase IX Protein (CA9-HM201)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CA9, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CA9 Antibody, hFc Tag with the EC50 of 11.3ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln38-Asp414

Catalog: CA9-HM201

$330.00
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Product Description Recombinant Human CA9/Carbonic Anhydrase IX Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Gln38-Asp414.

Product Background

CA9 is a member of the carbonic anhydrases' family, that is often expressed in cancer cells under hypoxic condition. CA9 expression potentially contributes to the regulation of cancer cell differentiation and mediates tumour-associated genes and signalling pathways, including apoptosis, hypoxia, G2M checkpoint, PI3K/AKR/mTOR signalling and TGF-beta signalling pathways.

Product Category

Recombinant Protein

Target

CA9/Carbonic Anhydrase IX

Gene/Protein ID

Q16790

Species

Human

Exact Sequence

Gln38-Asp414
Molecular Weight The protein has a predicted MW of 66.91 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human CA9, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CA9 Antibody, hFc Tag with the EC50 of 11.3ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Liquid
Shipping Shipped with dry ice.
Formulation Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human CA9, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CA9 Antibody, hFc Tag with the EC50 of 11.3ng/ml determined by ELISA.
Measured by its esterase activity. The specific activity is > 50 pmol/min/µg.
The purity of Human CA9 is greater than 95% as determined by SEC-HPLC.
Human CA9 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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