Recombinant Cas9 Protein, GMP Grade (GMP-CAS-EE110)

  • Quality Statement:
    Manufactured to the GMP guidelines

    Accepted by the FDA's Drug Master Files (DMF) database, DMF#036578
  • Express System: E.coli
  • Purity: > 95% as determined by Tris-Bis PAGE
    >95% as determined by HPLC
  • Exact Sequence: Asp2-Asp1368

Catalog: GMP-CAS-EE110

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Product Description DMF filed- this product has been submitted to FDA's Drug Master Files (DMFs) and is granted the DMF #036578.
Recombinant S. pyogenes CRISPR-Cas9 Protein (GMP grade) is expressed from E.coli for genome editing applications with CRISPR technology. It contains Asp2-Asp1368.
Product Category Recombinant Protein
Target Cas9
Synonyms CRISPR-associated endonuclease Cas9/Csn1; cas9; SpCas9; SpyCas9
Gene/Protein ID Q99ZW2
Species S. pyogenes
Exact Sequence Asp2-Asp1368
Molecular Weight The protein has a predicted MW of 163 kDa based on Tris-Bis PAGE result.
Express System E.coli
Purity > 95% as determined by Tris-Bis PAGE
>95% as determined by HPLC
Endotoxin Less than 0.01 EU perμg by the LAL method.
Quality Statement
Manufactured to the GMP guidelines

Accepted by the FDA's Drug Master Files (DMF) database, DMF#036578
Form Liquid
Shipping Shipped with blue ice
Formulation Supplied as 0.22 μm filtered solution in 30 mM Tris-HCl, 0.3 M NaCl, 50% Glycerol, 0.1 mM EDTA, pH7.4. Please dilute to the desired concentration according to the concentration of the solution shown on the product label.
Stability And Storage -20°C for 12 months. Avoid repeated freeze-thaw cycles.
Cas9 cuts the substrate DNA standard through the in vitro cutting experiment. The result of the figure shows that the cleavage activity of Kactus Cas9 is equivalent to that of other brands.
Cas9 is electrotransfected into 293T cell line in the form of RNP to knock out the target gene. The results show that Kactus Cas9 has the same knockout efficiency in cells as foreign brand.
Test samples which have 3-fold more fluorescence than the minus-DNase control should be considered contaminated with DNase,and the test result of Kactus Cas9 was negative.
Test samples which have 3-fold more fluorescence than the minus-RNase control should be considered contaminated with RNase,and the test result of Kactus Cas9 was negative.
ELISA assay is used for determining the residual host-cell proteins and the result is 3.0 ng/ml, which is far lower than the detection standard (<100 ng/ml).
The purity of Kactus Cas9 detected by SEC-HPLC is higher than 95%.
The purity of Kactus Cas9 detected by Tris-Bis-PAGE is higher than 95%.

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