|Product Description||The 7-methylguanosine cap structure(m7Gppp, Cap0) is a specific feature in eukaryotic mRNA, which is required for mRNA stability, splicing, nuclear export, effective translation, and mRNA decay. Vaccinia Capping Enzyme, a heterodimer of D1 and D12 subunits, adding the Cap0 structure to the 5' end of the RNA. The D1 subunit comprises an N-terminal RNA triphosphatase(TPase), a guanylyltransferase(GTase), and a C-terminal guanine-N7-methyltransferase(MTase). The D12 subunit binds and stimulates the MTase.
mRNA Cap 2'-O-Methyltransferase (Kactus #MEH-VE101) is required to generate Cap 1 structure that reduces cellular innate immune response when the RNA is used in vivo.
Expressed in an E.coli strain carries the genes of the Vaccinia virus Capping Enzyme.
In Vitro Synthesis(IVT)
Labeling 5' end of mRNA
|Shipping||Shipped with blue ice.|
|Stability And Storage||-20°C for 12 months. Avoid repeated freeze-thaw cycles.|