Vaccinia Capping Enzyme (VCS-VE101)
Catalog: VCS-VE101
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| Product Description | The 7-methylguanosine cap structure(m7Gppp, Cap0) is a specific feature in eukaryotic mRNA, which is required for mRNA stability, splicing, nuclear export, effective translation, and mRNA decay. Vaccinia Capping Enzyme, a heterodimer of D1 and D12 subunits, adding the Cap0 structure to the 5' end of the RNA. The D1 subunit comprises an N-terminal RNA triphosphatase(TPase), a guanylyltransferase(GTase), and a C-terminal guanine-N7-methyltransferase(MTase). The D12 subunit binds and stimulates the MTase. mRNA Cap 2'-O-Methyltransferase (Kactus #MEH-VE101) is required to generate Cap 1 structure that reduces cellular innate immune response when the RNA is used in vivo. Expressed in an E.coli strain carries the genes of the Vaccinia virus Capping Enzyme. |
| Product Category | Enzyme |
| Application | RNA Modification In Vitro Synthesis(IVT) Labeling 5' end of mRNA |
| Concentration | 10U/UL |
| Express System | E.coli |
| Form | Liquid |
| Shipping | Shipped with blue ice. |
| Stability And Storage | -20°C for 12 months. Avoid repeated freeze-thaw cycles. |
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