Biotinylated Cynomolgus IL-10 R alpha Protein (Primary Amine Labeling) (IL1-CM1RAB)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Human IL-10, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus IL-10 R alpha, His Tag with the EC50 of 49.8ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: His22-Asn233

Catalog: IL1-CM1RAB

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Product Description Recombinant Biotinylated Cynomolgus IL-10 R alpha Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-terminus.It contains His22-Asn233.

Product Background

 The interleukin-10 receptor alpha (IL10RA) gene codes for the alpha chain of the IL-10 receptor which binds the cytokine IL-10. IL-10 is an anti-inflammatory cytokine with immunoregulatory function during the pathogenesis of many inflammatory disorders in livestock, including Johne's disease (JD). JD is a chronic enteritis in cattle caused by Mycobacterium avium subsp. paratuberculosis (MAP) and is responsible for significant economic losses to the dairy industry. Several candidate genes including IL10RA have been found to be associated with JD.

Product Category

 Recombinant Protein

Target

 IL-10 R alpha

Gene/Protein ID

 A0A2K5WJ66

Species

 Cynomolgus

Exact Sequence

 His22-Asn233
Molecular Weight The protein has a predicted MW of 25.34 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized Human IL-10, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus IL-10 R alpha, His Tag with the EC50 of 49.8ng/ml determined by ELISA. See testing image for detail.

Product Tag

 C-His

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human IL-10, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus IL-10 R alpha, His Tag with the EC50 of 49.8ng/ml determined by ELISA.
The purity of Biotinylated Cynomolgus IL-10 R alpha is greater than 95% as determined by SEC-HPLC.
Biotinylated Cynomolgus IL-10 R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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