MHC-I Complexes

Check out our line of mammalian cell-derived MHC-I complexes including biotinylated and tetramer complexes.

T-Lymphocyte Activation by MHC

T lymphocytes, key mediators of the adaptive immune response, are activated when their T-cell receptors (TCRs) interact with cognate antigenic peptides displayed by major histocompatibility complex molecules (MHC).

Mammalian Expression

E. coli expression and in vitro protein refolding is a common method to produce MHC-I complexes. However, using our SAMS™ technology platform, KACTUS succeeded in expressing biotinylated MHC-I complex in the mammalian cell line Expi293.

High affinity, low endotoxin

The resulting MHC-I complexes show high affinities with benchmark antibodies and have low endotoxin levels – ideal for endotoxin sensitive cell-based assays. KACTUS also provides MHC tetramer complexes which have a greater avidity to T cells and more stable binding.

Kactus provides a series of MHC-polypeptide complex proteins with natural conformation and complete glycosylation modification, including monomers, tetramers, biotinylated tetramers, VLPs, etc., to meet the requirements of immunization, antibody screening, TCRT, blocking assay, SPR and other applications.

Validation Data

We take pride in the amount of time and resources we invest into product development. We test multiple protein configurations to ensure each of our final products has a design structure that will ensure peak performance and purity.

Figure 1. SPR assay (Biacore T200) using anti-hFc IgG capture, anti-NY-ESO-1 (HLA-A*02:01) antibody, and human NY-ESO-1 (HLA-A*02:01) tetramer as the analyte. The affinity constant is 0.15 nM.

Figure 2. The purity of Human & Mouse Chimeric LMP2 (HLA-A*02:01) was greater than 95% as determined by SEC-HPLC.

Figure 3. Immobilized Biotinylated Human GP100 (HLA-A*02:01), His Tag at 2μg/ml (100μl/Well). Dose response curve for Anti-GP100 (HLA-A*02:01) Ab., hFc tag with an EC50 of 0.3μg/mL determined by ELISA.

Catalog: MHC Complexes

View our full line of MHC complexes

HLA-G & LILRs

Learn more about our HLA-G and LILRB/LILRA products

Looking for something custom?

We are continuously building out our portfolio of MHC complexes and other products. If you can't find what you're looking for, let us know and we can create modifications on our current products or help you design a new one.