Biotinylated Human B7-H7/HHLA2 Protein (BH7-HM477B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human B7-H7, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD28H, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ile23-Asn344

Catalog: BH7-HM477B

$525.00
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Product Description Recombinant Biotinylated Human B7-H7/HHLA2 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ile23-Asn344.

Product Background

 B7-H7, also known as HHLA2 (HERV-H LTR-associating 2), is a member of the B7 family of immune regulatory proteins.Through interaction with TMIGD2, costimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade.

Product Category

 Recombinant Protein

Target

 B7-H7/HHLA2

Gene/Protein ID

 Q9UM44-1

Species

 Human

Exact Sequence

 Ile23-Asn344
Molecular Weight The protein has a predicted MW of 39.88 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized Biotinylated Human B7-H7, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD28H, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA. See testing image for detail.

Product Tag

 C-His-Avi

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Liquid
Shipping Shipped with dry ice.
Formulation Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human B7-H7, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD28H, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA.
The purity of Biotinylated Human B7-H7 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human B7-H7 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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