Biotinylated Human FGL1 Protein (FGL-HM411B)

Express System: HEK293
Product Tag: N-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE
Biotinylated: yes
Activity: Immobilized Biotinylated Human FGL1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LAG3, hFc Tag with the EC50 of 0.16ug/ml determined by ELISA. See testing image for detail.
Exact Sequence: Asp64-Asn305

Catalog: FGL-HM411B

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Product Description	Recombinant Biotinylated Human FGL1 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Asp64-Asn305.
Product Background	Fibrinogen-like protein 1 (FGL-1) is a protein that is structurally related to fibrinogen. In humans, FLG-1 is encoded by the FGL1 gene.Fibrinogen-like protein 1 is a member of the fibrinogen family of proteins, which also includes fibrinogen, fibrinogen-like protein 2, and clotting factors V, VIII, and XIII.Fibrinogen-like Protein 1 is a major immune inhibitory ligand of LAG-3.
Product Category	Recombinant Protein

Target	FGL1
Synonyms	FGL1; FGL-1; Hepassocin; HP-041; HFREP-1; LFIRE-1; HFREP1; Lag3 ligand
Gene/Protein ID	Q08830
Species	Human
Exact Sequence	Asp64-Asn305
Molecular Weight	The protein has a predicted MW of 31.9 kDa same as Tris-Bis PAGE result.
Activity	Immobilized Biotinylated Human FGL1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LAG3, hFc Tag with the EC50 of 0.16ug/ml determined by ELISA. See testing image for detail.
Product Tag	N-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Biotinylated Human FGL1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LAG3, hFc Tag with the EC50 of 0.16ug/ml determined by ELISA.
	Biotinylated Human FGL1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%..