Biotinylated Human HLA-A*03:01&B2M&gp100 (ALLAVGATK) Monomer Protein (MHC-HM463B)
Catalog: MHC-HM463B
Order now get it on
| Product Description | Recombinant Biotinylated Human HLA-A*03:01&B2M&gp100 (ALLAVGATK) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305 (HLA-A*03:01), Ile21-Met119 (B2M) and ALLAVGATK peptide. |
Product Background |
Immunization against the ALLAVGATK peptide may occur in melanoma patients and that the immunogenicity of this peptide is similar to that of the majority of melanoma differentiation antigens defined to date. Therefore, the use of ALLAVGATK in active immunotherapy for HLA-A3 melanoma patients, for whom no other immunogenic peptide has been so far described, remains a distinct possibility, providing that patients treated in vivo with this peptide develop a better in vitro response than already demonstrated for HLA-A2 epitopes generated from this same antigenic protein. |
Product Category |
Recombinant Protein |
Target |
HLA-A*03:01&B2M&gp100 (ALLAVGATK) Monomer |
| MHC Form | Monomer |
| Allele | HLA-A*03:01 |
Gene/Protein ID |
NP_002107.3(HLA-A*03:01)&P61769(B2M)&ALLAVGATK |
Species |
Human |
Exact Sequence |
Gly25-Thr305(HLA-A*03:01), Ile21-Met119(B2M) and ALLAVGATK peptide |
| Molecular Weight | The protein has a predicted MW of 50.15 kDa. Due to glycosylation, the protein migrates to 51-60 kDa based on Tris-Bis PAGE result. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
The purity of Biotinylated Human HLA-A*03:01&B2M&gp100 (ALLAVGATK) Monomer is greater than 95% as determined by SEC-HPLC. |
|
|
Biotinylated Human HLA-A*03:01&B2M&gp100 (ALLAVGATK) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
You may also be interested in
View more associated products