Biotinylated Human IL-1 alpha/IL-1A Protein (IL1-HM40AB)
Catalog: IL1-HM40AB
Order now get it on
| Product Description | Recombinant Biotinylated Human IL-1 alpha/IL-1A Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ser113-Ala271. |
Product Background |
The interleukin (IL)-1 family of cytokines is currently comprised of 11 members that have pleiotropic functions in inflammation and cancer. IL-1α and IL-1β were the first members of the IL-1 family to be described, and both signal via the same receptor, IL-1R. Over the last decade, much progress has been made in our understanding of biogenesis of IL-1β and its functions in human diseases. |
Product Category |
Recombinant Protein |
Target |
IL-1 alpha/IL-1A |
Gene/Protein ID |
P01583 |
Species |
Human |
Exact Sequence |
Ser113-Ala271 |
| Molecular Weight | The protein has a predicted MW of 20.95 kDa. Due to glycosylation, the protein migrates to 25-32 kDa based on Tris-Bis PAGE result. |
Activity |
The affinity constant of 0.76 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human IL-1R1, His Tag immobilized on CM5 Chip can bind Biotinylated Human IL-1 alpha, His Tag with an affinity constant of 0.76 uM as determined in SPR assay (Biacore T200). |
|
|
The purity of Biotinylated Human IL-1 alpha is greater than 95% as determined by SEC-HPLC. |
|
|
Biotinylated Human IL-1 alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
You may also be interested in
View more associated products