Biotinylated Human SKP1 Protein (SKP-HM401B)
Catalog: SKP-HM401B
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| Product Description | Recombinant Biotinylated Human SKP1 Protein is expressed from HEK293 His tag at the N-terminus and Avi tag at the C-terminusIt contains Pro2-Lys163. |
Product Background |
The S-phase Kinase-Associated Protein 1 (SKP1) is a core component of the SKP1, Cullin 1, F-box protein (SCF) complex, an E3 ubiquitin ligase that serves to poly-ubiquitinate a vast array of protein targets as a signal for their proteasomal degradation, thereby playing a critical role in the regulation of downstream biological processes. |
Product Category |
Recombinant Protein |
Target |
SKP1 |
Gene/Protein ID |
P63208-1 |
Species |
Human |
Exact Sequence |
Pro2-Lys163 |
| Molecular Weight | The protein has a predicted MW of 21.43 kDa. Due to glycosylation, the protein migrates to 23-28 kDa based on Tris-Bis PAGE result. |
Product Tag |
N-His,C-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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The purity of Biotinylated Human SKP1 is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human SKP1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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