Biotinylated Human TRAIL R1/DR4/TNFRSF10A Protein (TRL-HM4R1B)
Catalog: TRL-HM4R1B
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| Product Description | Recombinant Biotinylated Human TRAIL R1/DR4/TNFRSF10A Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Pro34-Asn239. |
Product Background |
Tumor necrosis factor receptor superfamily member 10A (TNFRSF10A) is also known as TNF-related apoptosis-inducing ligand receptor 1 (TRAIL-R1), Death receptor 4 (DR4), CD261 and APO2, which belongs to TNF superfamily.The expression of apoptosis-inducing TRAIL-R1 and TRAIL-R2 and of the decoy receptors TRAIL-R3 and TRAIL-R4 was systematically studied in all developmental stages of peripheral B cells isolated from the blood and secondary lymphoid organs. Expression of TRAIL-Rs is modulated along development, with highest levels observed in germinal center B cells. |
Product Category |
Recombinant Protein |
Target |
TRAIL R1/DR4/TNFRSF10A |
| Synonyms | DR4; CD261; TNFRSF10A; TRAIL-R; APO2; TRAIL R1; MGC9365 |
Gene/Protein ID |
O00220 |
Species |
Human |
Exact Sequence |
Pro34-Asn239 |
| Molecular Weight | The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Human TRAIL, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TRAIL R1, His Tag with the EC50 of 0.31ug/ml determined by ELISA (QC Test). The affinity constant of 0.315 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Biotinylated Human TRAIL R1, His-Avi Tag immobilized on CM5 Chip can bind Human TRAIL, No Tag with an affinity constant of 0.315 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human TRAIL, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TRAIL R1, His Tag with the EC50 of 0.31ug/ml determined by ELISA (QC Test). |
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The purity of Biotinylated Human TRAIL R1 is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human TRAIL R1 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%. |
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