Biotinylated Rhesus macaque CD5 Protein (CD5-RM405B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Rhesus macaque CD5, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CD5 Antibody, hFc Tag with the EC50 of 9.3ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Arg25-Pro375

Catalog: CD5-RM405B

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Product Description Recombinant Biotinylated Rhesus macaque CD5 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Arg25-Pro375.

Product Background

CD5: a type I transmembrane protein found on T cells, thymocytes, and some B cells that is a ligand for CD72 and is involved in cellular activation or adhesion; expressed in B-cell chronic lymphocytic leukemia and T-cell lymphoma.

Product Category

Recombinant Protein

Target

CD5
Synonyms CD5 molecule; CD5; LEU1; T1; CD5 antigen

Gene/Protein ID

F6V5Q9

Species

Rhesus macaque

Exact Sequence

Arg25-Pro375
Molecular Weight The protein has a predicted MW of 41.7 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Biotinylated Rhesus macaque CD5, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CD5 Antibody, hFc Tag with the EC50 of 9.3ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Rhesus macaque CD5, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CD5 Antibody, hFc Tag with the EC50 of 9.3ng/ml determined by ELISA.
Biotinylated Rhesus macaque CD5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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