Cynomolgus B7-H7/HHLA2 Protein (BH7-CM177)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 7.86 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Ile21-Asn345

Catalog: BH7-CM177

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Product Description	Recombinant Cynomolgus B7-H7/HHLA2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile21-Asn345.
Product Background	B7-H7, also known as HHLA2 (HERV-H LTR-associating 2), is a member of the B7 family of immune regulatory proteins.Through interaction with TMIGD2, costimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade.
Product Category	Recombinant Protein

Target	B7-H7/HHLA2
Gene/Protein ID	XP_005548285.2
Species	Cynomolgus
Exact Sequence	Ile21-Asn345
Molecular Weight	The protein has a predicted MW of 38.31 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Activity	The affinity constant of 7.86 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Cynomolgus B7-H7, His Tag captured on CM5 Chip via anti-his antibody can bind Human CD28H, hFc Tag with an affinity constant of 7.86 nM as determined in SPR assay (Biacore T200).
	The purity of Cynomolgus B7-H7 is greater than 95% as determined by SEC-HPLC.
	Cynomolgus B7-H7 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.