Cynomolgus IL-8/CXCL8 Protein (IL8-CM108)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Cynomolgus IL-8, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-8 Antibody, hFc Tag with the EC50 of 13.0ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ala23-Pro100

Catalog: IL8-CM108

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Product Description	Recombinant Cynomolgus IL-8/CXCL8 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala23-Pro100.
Product Background	Interleukin-8 (IL-8) has been revealed as a critical regulator of CNS function and development with participation in many CNS disorders including gliomas.Several promising approaches that target directly or indirectly IL-8 effects in gliomas are currently in progress while more-in-depth studies are needed to validate its biomarker role and elucidate the underlying molecular mechanisms.
Product Category	Recombinant Protein

Target	IL-8/CXCL8
Gene/Protein ID	A0A2K5TUL7
Species	Cynomolgus
Exact Sequence	Ala23-Pro100
Molecular Weight	The protein has a predicted MW of 10.12 kDa. Due to glycosylation, the protein migrates to 12-14 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Cynomolgus IL-8, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-8 Antibody, hFc Tag with the EC50 of 13.0ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Cynomolgus IL-8, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-8 Antibody, hFc Tag with the EC50 of 13.0ng/ml determined by ELISA.
	Cynomolgus IL-8 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.