Non-biotinylated Human BCMA/TNFRSF17 Protein, Ultra Low Endotoxin (BCM-HM117-UL)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human BCMA, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 10.1 ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Met1-Ala54
Catalog #BCM-HM117-UL
Regular price $363.00
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Product Description

Recombinant Human BCMA/TNFRSF17 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met1-Ala54.

Product Background

B-cell maturation antigen (BCMA or BCM), also known as tumor necrosis factor receptor superfamily member 17 (TNFRSF17), is a protein that in humans is encoded by the TNFRSF17 gene. TNFRSF17 is a cell surface receptor of the TNF receptor superfamily which recognizes B-cell activating factor (BAFF).

Product Category

Recombinant Protein / Antibody discovery / CAR-T targets
Protein
BCMA/TNFRSF17
Synonyms
CD269; TNFRSF17; BCMA; BCM; TNFRSF13A
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Met1-Ala54
Molecular Weight
The protein has a predicted MW of 8.9 kDa. Due to glycosylation, the protein migrates to 15-25 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human BCMA, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 10.1 ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 0.01 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human BCMA, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 10.1 ng/ml determined by ELISA (QC Test). Immobilized Human BCMA, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 10.1 ng/ml determined by ELISA (QC Test).
Immobilized Human BCMA, His Tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-BCMA Antibody, hFc Tag with the EC50 of 2.4 ng/ml determined by ELISA. Immobilized Human BCMA, His Tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-BCMA Antibody, hFc Tag with the EC50 of 2.4 ng/ml determined by ELISA.
Human BCMA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human BCMA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Biotinylated Human BAFF, His-Avi Tag on Streptavidin-Biosensor can bind Human BCMA, His Tag with an affinity constant of 0.65 µM as determined in BLI assay (Gator® Prime). Loaded Biotinylated Human BAFF, His-Avi Tag on Streptavidin-Biosensor can bind Human BCMA, His Tag with an affinity constant of 0.65 µM as determined in BLI assay (Gator® Prime).
Loaded Anti-BCMA antibody on ProA-Biosensor can bind Human BCMA, His Tag with an affinity constant of 16.10 nM as determined in BLI assay (Gator® Prime). Loaded Anti-BCMA antibody on ProA-Biosensor can bind Human BCMA, His Tag with an affinity constant of 16.10 nM as determined in BLI assay (Gator® Prime).
The purity of Human BCMA is greater than 95% as determined by SEC-HPLC. The purity of Human BCMA is greater than 95% as determined by SEC-HPLC.
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