Phi29 DNA Polymerase (PHI-BE101)
Catalog: PHI-BE101
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| Product Description | Phi29 DNA polymerase is a DNA polymerase cloned from Bacillus subtilis phage phi29, with strong strand displacement activity and high processivity (up to more than 70 kbp). The enzyme has inherent 3'-5' exonuclease activity and higher fidelity than most Taq enzymes. It is used for isothermal amplification in vitro. |
| Synonyms | Phi29 enzyme; Phi29 protein; Phi29 polymerase; ϕ29 dna polymerase; ϕ29 polymerase; ϕ29 enzyme |
| Application | Multiple strand displacement amplification (MDA) Whole genome amplification (WGA) Rolling circle amplification |
| Concentration | 10U/µL |
| Unit Definition | One unit is defined as the amount of enzyme required to incorporate 0.5 pmol of dNTPs into acid-insoluble substances within 10 minutes in a total reaction volume of 50µL at 30°C. |
| Source | E. coli strain carrying the phi29 DNA polymerase gene cloned from Bacillus subtilis phage phi29 |
| Form | Liquid |
| Shipping | Shipped on dry ice. |
| Stability And Storage | -20±5°C for 24 months. Avoid repeated freeze-thaw cycles. |
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Rolling cycle amplification of pUC57 plasmid with phi29 DNA Polymerase, product was digested with BspQI and then analyzed with Agarose gel. |
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Phi29 DNA Polymerase on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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