Exonuclease III (EXO-EE101)
  • Description: Catalyzes the removal of nucleotides from linear or nicked double stranded DNA in the 3’ to 5’ direction
  • Applications: Site-directed mutagenesis
    Preparation of nested deletions in dsDNA
    Preparation of strand-specific probes
    Preparation of ssDNA for dideoxy-sequencing
  • Related Products:

    Phi29 DNA Polymerase (#PHI-BE101)

    TelN Protelomerase (#TLN-BE001)

Catalog #EXO-EE101
Regular price $60.00
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Product Description
Exonuclease III is a double-stranded DNA-specific exonuclease. Exonuclease III catalyzes the removal of nucleotides from linear or nicked double stranded DNA in the 3’ to 5’ direction. The degradation of Exonuclease III could be initiated from 3’ blunt end, 3’ recessed end, 3’ overhangs with less than 4 bases and nicked DNA.
Synonyms
ExoIII; Exo III;
Applications
Site-directed mutagenesis
Preparation of nested deletions in dsDNA
Preparation of strand-specific probes
Preparation of ssDNA for dideoxy-sequencing
Concentration
100U/µL
Unit Definition
One unit is defined as the amount of enzyme required to produce 1 nmol of acid-soluble total nucleotides in 30 minutes at 37°C in a total reaction volume of 50μL.
Source
E. coli strain that carries E. coli Exonuclease III gene
Biotinylated
no
Form
Liquid
Shipping
Shipped with dry ice
Stability And Storage
-20±5°C for 24 months. Avoid repeated freeze-thaw cycles.
Digestion of 1μg linearized plasmid using Exonuclease III, followed by Agarose gel analysis.
Exonuclease III on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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