• Description: Catalyzes the removal of nucleotides from linear or nicked double stranded DNA in the 3’ to 5’ direction
  • Applications: Site-directed mutagenesis
    Preparation of nested deletions in dsDNA
    Preparation of strand-specific probes
    Preparation of ssDNA for dideoxy-sequencing
Catalog #EXO-EE101
Price
Regular price $6600
Regular price Sale price $6600
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Exonuclease III (EXO-EE101) Summary

Product Description

 Exonuclease III is a double-stranded DNA-specific exonuclease. Exonuclease III catalyzes the removal of nucleotides from linear or nicked double stranded DNA in the 3’ to 5’ direction. The degradation of Exonuclease III could be initiated from 3’ blunt end, 3’ recessed end, 3’ overhangs with less than 4 bases and nicked DNA.

Exonuclease III (EXO-EE101) Specifications/Details

Synonyms
 ExoIII; Exo III;
Applications
 Site-directed mutagenesis
Preparation of nested deletions in dsDNA
Preparation of strand-specific probes
Preparation of ssDNA for dideoxy-sequencing
Concentration
 100U/µL
Unit Definition
 One unit is defined as the amount of enzyme required to produce 1 nmol of acid-soluble total nucleotides in 30 minutes at 37°C in a total reaction volume of 50μL.
Source
 E. coli strain that carries E. coli Exonuclease III gene
Biotinylated
 no

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice
Stability And Storage
 -20±5°C for 24 months. Avoid repeated freeze-thaw cycles.

Validation Images

Digestion of 1μg linearized plasmid using Exonuclease III, followed by Agarose gel analysis. Digestion of 1μg linearized plasmid using Exonuclease III, followed by Agarose gel analysis.
Other Available Proteins:
Finding available proteins...