Exonuclease III (EXO-EE101)

  • Description: Catalyzes the removal of nucleotides from linear or nicked double stranded DNA in the 3’ to 5’ direction
  • Applications: Site-directed mutagenesis
    Preparation of nested deletions in dsDNA
    Preparation of strand-specific probes
    Preparation of ssDNA for dideoxy-sequencing

Catalog: EXO-EE101

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Product Description Exonuclease III is a double-stranded DNA-specific exonuclease. Exonuclease III catalyzes the removal of nucleotides from linear or nicked double stranded DNA in the 3’ to 5’ direction. The degradation of Exonuclease III could be initiated from 3’ blunt end, 3’ recessed end, 3’ overhangs with less than 4 bases and nicked DNA.
Application Site-directed mutagenesis
Preparation of nested deletions in dsDNA
Preparation of strand-specific probes
Preparation of ssDNA for dideoxy-sequencing
Concentration 100U/µL
Unit Definition One unit is defined as the amount of enzyme required to produce 1 nmol of acid-soluble total nucleotides in 30 minutes at 37°C in a total reaction volume of 50μL.
Source E. coli strain that carries E. coli Exonuclease III gene
Form Liquid
Shipping Shipped with dry ice
Stability And Storage -20±5°C for 24 months. Avoid repeated freeze-thaw cycles.
Exonuclease III structure.

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