Amplification with low starting quantities
Phi29 DNA polymerase is a highly processive enzyme that can efficiently amplify DNA sequences in vitro. It is often used as an alternative to the more commonly used polymerase chain reaction (PCR) method for DNA amplification. Phi29 DNA polymerase amplification has several advantages over PCR, including higher processivity, reduced reaction time, and higher fidelity. It is particularly useful for amplifying DNA from samples with limited starting material, such as ancient DNA or single cells. It is a highly robust enzyme and is suitable for rolling circle amplification (RCA), multiple-displacement amplification (MDA), and whole genome amplification.
TelN Protelomerase cleaves dsRNA at its recognition site and leaves covalently closed ends. It can be used for DNA vaccine development as well as for non-viral therapy vectors. TelN Protelomerase is isolated from phage N15.
Exonuclease III is a highly processive enzyme that hydrolyzes the phosphodiester bonds in DNA in the 3' to 5' direction, resulting in stepwise removal of nucleotides. Exonuclease III catalyzes the removal of nucleotides from linear or nicked double stranded DNA. Degradation of Exonuclease III could be initiated from a 3’ blunt end, 3’ recessed end, 3’ overhangs with less than 4 bases and nicked DNA.