TelN Protelomerase (TLN-BE001)

Catalog: TLN-BE001


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Product Description TelN Protelomerase is cloned from bacteriophage N15. TelN cuts dsDNA at the recognition site TelRL (56 bp), which consists of a palindromic sequence-TelO in the middle and palindromic sequences R3 and L3 at both ends of 14 bp form. TelN has cutting-ligating enzyme activity and covalently ligates at the cleavage site forming hairpin termini.
Synonyms TelN; Phage N15 protelomerase; Protelomerase; TelN enzyme; TelN protein;
Application In vitro enzymatic synthesis of DNA constructs
Concentration 10U/µL
Unit Definition One unit is defined as the amount of enzyme required to cut 0.5 μg completely BsaI linearized pMiniT-TelN control plasmid (313 fmol TelN recognition site) at 30°C for 30 min in a total reaction volume of 20 μL.
Source E.coli strain that carries TelN from phage N15
Form Liquid
Shipping Shipped with dry ice
Stability And Storage -20±5°C for 12 months. Avoid repeated freeze-thaw cycles.
TelN Protelomerase cut substrate DNA containing 513 fmol cleaveage site, followed by agarose gel analysis.
TelN Protelomerase on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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