TelN Protelomerase (TLN-BE001)
Catalog: TLN-BE001
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| Product Description | TelN Protelomerase is cloned from bacteriophage N15. TelN cuts dsDNA at the recognition site TelRL (56 bp), which consists of a palindromic sequence-TelO in the middle and palindromic sequences R3 and L3 at both ends of 14 bp form. TelN has cutting-ligating enzyme activity and covalently ligates at the cleavage site forming hairpin termini. |
| Synonyms | TelN; Phage N15 protelomerase; Protelomerase; TelN enzyme; TelN protein; |
| Application | In vitro enzymatic synthesis of DNA constructs |
| Concentration | 10U/µL |
| Unit Definition | One unit is defined as the amount of enzyme required to cut 0.5 μg completely BsaI linearized pMiniT-TelN control plasmid (313 fmol TelN recognition site) at 30°C for 30 min in a total reaction volume of 20 μL. |
| Source | E.coli strain that carries TelN from phage N15 |
| Form | Liquid |
| Shipping | Shipped with dry ice |
| Stability And Storage | -20±5°C for 12 months. Avoid repeated freeze-thaw cycles. |
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TelN Protelomerase cut substrate DNA containing 513 fmol cleaveage site, followed by agarose gel analysis. |
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TelN Protelomerase on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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