Product Description |
TelN Protelomerase is cloned from bacteriophage N15. TelN cuts dsDNA at the recognition site TelRL (56 bp), which consists of a palindromic sequence-TelO in the middle and palindromic sequences R3 and L3 at both ends of 14 bp form. TelN has cutting-ligating enzyme activity and covalently ligates at the cleavage site forming hairpin termini. |
Synonyms |
TelN; Phage N15 protelomerase; Protelomerase; TelN enzyme; TelN protein; |
Applications |
In vitro enzymatic synthesis of DNA constructs |
Concentration |
10U/µL |
Unit Definition |
One unit is defined as the amount of enzyme required to cut 0.5 μg completely BsaI linearized pMiniT-TelN control plasmid (313 fmol TelN recognition site) at 30°C for 30 min in a total reaction volume of 20 μL. |
Source |
E.coli strain that carries TelN from phage N15 |
Biotinylated |
no |
Form |
Liquid |
Shipping |
Shipped with dry ice |
Stability And Storage |
-20±5°C for 12 months. Avoid repeated freeze-thaw cycles. |
|
|
TelN Protelomerase cut substrate DNA containing 513 fmol cleaveage site, followed by agarose gel analysis. |
|
|
TelN Protelomerase on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recently viewed
