• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Asp1-Ala144
Catalog #TRB-HM2C1
Price
Regular price $33000
Regular price Sale price $33000
/
Size
Avantor VWR Fisher Scientific

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Human TRBC1 Protein (TRB-HM2C1) Summary

Product Description

 Recombinant Human TRBC1 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Asp1-Ala144.

Product Background

 T cell receptor β-chain constant (TRBC) is a promising class of cancer targets consisting of two highly homologous proteins, TRBC1 and TRBC2. While each TCRα/β carries either TRBC1 or TRBC2 in a mutually exclusive manner, the normal T cell population comprises a mixture of individual cells, either expressing TRBC1 or TRBC2. However, the cancer T-cell population exclusively expresses either TRBC1 or TRBC2. Using antibodies to specifically target TRBC1 (TCRαβ1) or TRBC2 (TCRαβ2) (in the case of a TRBC1+ T cell malignancy or the case of a TRBC2+ malignancy, respectively) has been proposed to be a strategy to eradicate all cells of malignant cancer.

TRBC1 Specifications

Protein
 TRBC1
Synonyms
 T cell receptor beta constant 1
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Asp1-Ala144
Molecular Weight
 The protein has a predicted MW of 42.14 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result.
Product Tag
 C-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human TRBC1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human TRBC1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human TRBC1 is greater than 95% as determined by SEC-HPLC. The purity of Human TRBC1 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...