GMP-Grade
GMP-Grade & GMP-Ready
Enzymes for mRNA Production
Template Preparation, Transcription, & Modification
T7 RNA Polymerase
Our GMP-grade T7 RNA polymerase is engineered to provide high mRNA yield and accurate transcription of mRNA from DNA templates. It is designed to minimze dsRNA contaminzation in transcripts and is free of animal-free of antiobiotics and animal-derived products. Our T7 polymerase has been successfully submitted to the FDA Drug Master Files (#037660).
Restriction Enzymes
Restriction enzymes are used in mRNA synthesis to cleave DNA templates at sequence-specific sites. The enzymes introduce specific and well-defined breaks in the DNA, which then allow for the transcription of RNA with defined lengths and sequences.
KACTUS offers various GMP-Grade and GMP-Ready restriction enzymes. Learn more about a specific enzyme below.
GMP-Ready
GMP-Ready
GMP-Ready
GMP-Ready
Capping Enzymes
Vaccinia capping enzyme is a type of RNA capping enzyme that adds a 7-methylguanosine cap (m7G or m7Gppp) to the 5'-end of an mRNA molecule. This creates the Cap0 structure which protects the mRNA from degradation and is important for efficient translation and export of the mRNA from the nucleus to the cytoplasm.
mRNA cap 2'-O-methyltransferase adds a methyl group to the first nucleotide at the 2'-O position of ribose, resulting in the creation of the Cap1 strcuture (m7GpppNm). This 2'-O-methylation plays a role in authorizing foreign RNA as "self RNA" and hence in the non-self discrimination of the innate immune response against the mRNA.
KACTUS provides GMP-Grade Vaccinia Capping Enzyme and mRNA Cap 2'-O-Methyltransferase designed for use in a one-step capping reaction system to generate the Cap1 structure. Our capping enzymes demonstrate greater than 95% capping efficiency.
E. Coli Poly(A) Polymerase Tailing Enzyme
Poly(A) polymerase plays a crucial role in mRNA polyadenylation. The enzyme catalyzes the addition of adenosine monophosphates (AMPs) to the 3' end of the mRNA molecule, creating what is known as the poly(A) tail. The poly(A) tail protects RNA from degradation by nucleases, assists in the transport of RNA out of the nucleus, and stimulates translation of the RNA into protein.
Cyclization Enzymes
Cyclization enzymes are responsible for forming a unique 5'-5' phosphodiester bond between the first and last nucleotides of the mRNA molecule, creating what is known as a circular mRNA. This circularization process is important for increasing the stability and translational efficiency of the mRNA molecule, as well as protecting it from degradation by cellular exonucleases.
RNase R digests linear RNA.
RNase R leaves circular RNA intact.