Accurate AAV ELISA Kits for Reliable Results

A familiar protocol for easy execution.

Our AAV Titration ELISA kits use a standard sandwich ELISA method utilizing exclusive AAV antibodies developed using our proprietary immunization scheme.

Pre-coated microtiter plate with removable single-use test strips.
AAV standard and sample is incubated on plate.
Biotinylated detection antibody is added to bind AAV capsids.
Streptavidin HRP conjugate is added to bind with antibody-AAV complex.
HRP will then oxidize TMB substrate to produce a color change porportional to AAV capsid titer.

Proprietary monoclonal antibodies developed using enhanced immunization scheme.

Our AAV Titration ELISA kits using recombinant monoclonal antibodies developed using our proprietary immunization scheme that overcomes the weak AAV immunogenicity. This method maintains a remarkable level of consistency as the serum titers within the same group of mice consistently align. Across all AAV serotypes, our technique consistently elevates AAV anti-serum titers, resulting in the production of high- performance monoclonal antibodies.

AAV2 Titration ELISA Kit Exhibits Near-Zero Cross-reactivity with AAV3

This graph demonstrates the cross-reactivity analysis of the AAV2 Titration ELISA Kit with AAV3. The data shows a dose-response curve for the AAV2 standard, while the AAV3 standard exhibits minimal reactivity, indicating the kit's high specificity for AAV2 with no cross-reactivity detected for AAV3.

AAV2 Titration ELISA Kit

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Product Advantages

Wide Linear Range

Example 8-point standard curve for the AAV9 Titration ELISA Kit.

The quantitative range of this kit is 2.03x107 - 1.30109 capsids/mL.

Batch Consistency

KACTUS AAV9 Titration ELISA kit standard curve tested across two batches.

The standard curve is highly reproducible and exhibits an R²>0.99.

Specificity to intact capsids

The ELISA sandwich assay was used to detect the binding of AAV9 antibodies to intact and denatured AAV9 capsid proteins.

The AAV9 detection antibody does not exhibit binding to denatured AAV9 capsid protein.

Specificity to individual serotypes

The cross-binding of different concentrations of AAV9 antibodies to capsid proteins of other serotypes (AAV2/5/8/9) was detected by indirect ELISA.

The AAV9 titration ELISA kit is highly specific to the AAV9 serotype and does not exhibit cross-reactivity with other AAV serotypes.

High Accuracy

KACTUS AAV9 Titration ELISA kit was used to test the recovery rate of three AAV9 titers (high, medium, and low) in spiked dilution buffer (left) and HEK293 cell lysate (right).

The sample recovery rates of AAV9 samples with different titers detected in the dilution buffer and cell lysate were all between 80% and 120%, indicating that this kit has high accuracy in detecting AAV9 titers.

High Precision

Sample	Runs	Average (capsids/mL)	Coefficient of Variation (%CV)
6.5E+08 capsids/mL	n=10	6.18E+08	2.80%
1.63E+08 capsids/mL	n=10	1.92E+08	1.90%
2.03E+07 capsids/mL	n=10	2.17E+07	5.90%

The same batch of AAV9 test kits were used to test three different titer AAV9 samples (high, medium and low). Each sample was tested 10 times. The average and coefficient of variation (%CV) of these 10 titer tests was calculated.

The %CV for all three sample concentrations (high, medium and low) was less than 10%, indicating that the AAV9 Titration ELISA kit has good intra-batch repeatability.