Custom MHC Expression Services
At KACTUS, we specialize in providing high-quality and reliable custom MHC class I and MHC class II complexes to meet your immune monitoring needs. With our extensive expertise in manufacturing MHC tetramers, monomers, and peptide-ready MHCs, we ensure that each product is tailored to your specific requirements.
We understand that accurate antigen presentation is fundamental in immunotherapy research. Our platform supports both Class I MHC and other species alleles and is compatible with T cell receptor detection workflows. Whether your study involves T cells, TCRs, or TCR-mimic antibodies, we offer flexible MHC configurations to support diverse experimental models.
Service Highlights
Monomers & Tetramers
Biotinylation & Fluorescence
Class I, and Other Species Alleles
Mammalian or E. coli Expression
6-8 Week Production
SPR Binding Analysis for validating cell surface proteins interaction with T cells
Custom MHC Expression Workflow
1. Project Outline
After you submit your request, we'll set up an initial discussion to see what specifications you're looking for and your applications.
2. MHC Expression
We use structural information and our expertise in MHC engineering to produce mammalian-expressed single-chain trimer MHCs or peptide-ready MHCs.
3. Quality Control
Your product will undergo purity and activity testing depending on the specifications outlined for your application. This may include HPLC, ELISA, or SPR testing services.
4. Delivery
We'll deliver the final protein construct and any requested quality data in 6-8 weeks.
Available Alleles
Human MHC Class I
- HLA-A*01:01
- HLA-A*02:01
- HLA-A*02:03
- HLA-A*02:06
- HLA-A*03:01
- HLA-A*11:01
- HLA-A*24:02
- HLA-A*26:01
- HLA-A*31:01
- HLA-A*33:03
- HLA-A*68:01
- HLA-B*07:02
- HLA-B*15:01
- HLA-B*27:05
- HLA-B*57:01
- HLA-C*03:04
- HLA-C*07:02
- HLA-E*01:03
- HLA-G
Mouse MHC Class I
- H-2K
- Qa-1b
Other Alleles
For MHC Class II alleles, non-canonical alleles or alleles from other species, please submit our custom MHC request form and we will conduct a feasibility assessment for you.
Custom MHC Product Types
Monomers
Bacteria-refolded MHC or Mammalian-expressed SCT monomers
Tetramers
MHC tetramers for enhanced TCR avidity.
Peptide-ready MHC
Stable, peptide-free MHC for simple loading of your own antigenic peptide
Chimeric MHCs
Human-murine chimeric MHC for efficient TCR-mimic antibody generation
Example Data
Chimeric MHCs
We have creatively engineered species-chimeric MHC-peptide complexes, using a special design to replace MHC α3 or β2M with the corresponding domains from other species. This approach leaves more immunogenicity to the antigenic peptide, increasing the production of effective antibodies, thereby optimizing the screening process and reducing the screening intensity. Our chimeric MHCs are expressed in mammalian cells to maintain their natural conformation and demonstrate good bioactivity.
Figure 1. ELISA verification demonstrates that the Chimeric HLA-A02:01(mα3)&mB2M&NY-ESO-1 (SLLMWITQC) Monomer can bind to the HLA-A02:01&B2M&NY-ESO-1 (SLLMWITQC) Antibody. The activity is comparable to that of the non-chimeric HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer.
Figure 2. ELISA validation shows that the Chimeric HLA-A02:01(mα3)&mB2M&GP100 (YLEPGPVTA) Monomer can bind to the GP100 TCR&Anti-CD3 Bispecific Fusion. Its activity is comparable to the non-chimeric HLA-A02:01&B2M&GP100 (YLEPGPVTA) Monomer and superior to that of the chimeric monomer without special design.
Peptide-ready MHC
The discovery and identification of new antigens is an important process in immunotherapy. A key step in this process is detecting binding affinities of antigenic peptides with MHCs and the reactivity to TCRs. We have engineered functional Peptide-ready MHCs (prMHCs), which stabilized, peptide-free MHC monomers or tetramers. These prMHCs are available catalog and custom in Class I and Class II alleles.
Figure 3. Flow cytometry analysis of PE-labeled HLA-A02:01&B2M tetramers binding to Jurkat cells. TCR knockout Jurkat cells showed no significant binding to the tetramers, regardless of peptide presence. In contrast, 1G4 TCR monoclonal Jurkat cells exhibited high specific binding when the tetramers were complexed with the NY-ESO-1 (SLLMWITQC) peptide, while low binding was observed with an irrelevant peptide. This indicates that the loaded prMHC tetramer has high binding affinity with the TCR-expressing cells.
Webinar: Engineering MHCs as Versatile Reagents
Discover more about our MHC engineering by listening to our Chief Technology Officer, ManHee Suh, as he explains the innovative design of our diverse MHC molecules. Learn how we integrate novel peptide-loading strategies to support antigen processing, cell mediated immunity, and T cell receptor engineering across research involving MHC class I complexes, MHC class II complexes, and custom peptide sequence validation.
Why choose KACTUS?
Expertise in MHC Engineering
With extensive experience in MHC expression and engineering, our technical team specializes in producing customized MHC molecules for a wide range of research and therapeutic applications, utilizing our deep understanding of protein structure and function to ensure optimal stability and biological activity.
Highly Flexible MHC Customization
MHCs can be customized with various options, including biotinylation, fluorescent labels, and specific tags. We support a wide range of alleles and peptides, and products can be expressed in either mammalian or E. coli systems to meet your specific research needs.
Bioactivity Validation and Quality Control
Each MHC we produce undergoes quality control validation to ensure structural integrity and functional activity. Our quality control processes include cell sorting, binding assays, and purity testing to give you full confidence in the product quality.
Transparent and Responsive Scientific Support
With KACTUS, you can work with scientists who understand your challenges and care about your results. We’re committed to transparent communication, timely updates, and collaborative problem-solving so you can move forward with clarity and confidence.
Explore our most popular custom solutions
Soluble TCR Expression
Soluble TCR Expression services with engineering, and optimization of various formats of TCRs.
SPR Analysis Services
Characterization of biomolecular interactions using Surface Plasmon Resonance technology.
Solute Carrier Proteins
Custom solute carrier protein expression services displayed on VLPs or Nanodiscs.
MHC Complex Custom Service FAQs
Custom MHC molecules are essential tools in immune monitoring, T cell response studies, and antigen discovery. They help identify antigen-specific TCR interactions, making them valuable in immunotherapy research and vaccine development.
We produce MHC monomers, tetramers, peptide-ready MHCs, and chimeric MHCs, all tailored for specific research applications. Formats include biotinylation, fluorescent labeling, and species-specific domain replacements to reduce off-target effects.
We offer a wide selection of Class I, including HLA-A, HLA-B, HLA-C, HLA-E, HLA-G (human) and H-2K, Qa-1b (mouse). Custom alleles from other species can also be requested pending feasibility assessment. We do not currently offer Class II.
We’ll ask for details such as the MHC class, allele type, peptide sequence (if known), desired tags or labels, and your application. This helps us assess feasibility and align with your research goals from the outset.
The standard production timeline is 6 to 8 weeks including shipping, depending on construct complexity, expression system, and any custom labeling or testing requirements.
We offer mammalian expression for natural protein folding (includes PTMs) and E. coli expression for native sequence with no PTMs. The choice depends on the structure, application, and performance requirements of the molecule.
Yes. Products undergo purity and bioactivity validation, which may include ELISA, HPLC, FACS, or SPR analysis, depending on your project specifications.
Peptide-ready MHCs are stabilized, peptide-free MHC monomers or tetramers that allow researchers to load their own antigenic peptides in-house. They’re ideal for antigen screening and TCR specificity studies.
Chimeric MHCs feature species-specific domain substitutions (e.g., human α3 replaced with mouse α3) to reduce non-specific antibody binding. They're especially useful in antibody generation and screening workflows.
Yes. All project-related data, including peptides, sequences, and final constructs, remain confidential and fully owned by you. We also accommodate confidentiality agreements when required.
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60 Hickory Drive
Waltham, MA 02451
United States
Custom MHC Complex Service | Monomers & Tetramers | KACTUS