TCR Service Overview
We're pleased to offer specialized custom services related to MHC and TCR research, including the expression of TCRs, and TCR-related drugs. Researchers often need TCRs in order to study their role in immune responses, to develop TCR-based therapies, and to screen for potential therapeutic candidates. In support of this, we offer custom expression services for soluble TCRs and soluble TCR fusion proteins. Additionally, we can perform analysis services including matured affinity TCR ELISAs and low affinity TCR SPR assays.
TCR Service Features
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Production of various formats of soluble TCRs, including scFv-TCR, TCR-His, etc.
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TCR engineering to optimize soluble TCR expression based on TCR modeling
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SPR or ELISA analysis of soluble TCR & MHC interactions
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Case Studies
GP100 TCR & Anti-CD3 Bispecific Fusion
We have successfully expressed a GP100 TCR & Anti-CD3 Bispecific Fusion, which can not only bind to HLA-A*02:01&B2M&GP100 (YLEPGPVTA) complex, but also to CD3 heterodimers at the same time, demonstrating good bispecific binding activity.
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Figure 1. In SPR analysis, the GP100 TCR & Anti-CD3 Bispecific Fusion can bind to the HLA-A*02:01&B2M&GP100 (YLEPGPVTA) complex tetramer, with an affinity constant of 0.196 nM.
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Figure 2. ELISA verification demonstrates that GP100 TCR & Anti-CD3 Bispecific Fusion can bind to HLA-A*02:01&B2M&GP100 (YLEPGPVTA) complex monomer with an EC50 of 11.8 ng/mL.
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Figure 3. ELISA verification demonstrates that GP100 TCR & Anti-CD3 Bispecific Fusion can bind to Human CD3E&CD3D, with an EC50 value of 8.7 ng/mL.
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Figure 4. ELISA verification demonstrates that GP100 TCR & Anti-CD3 Bispecific Fusion can bind to Human CD3E&CD3G, with an EC50 value of 0.22 μg/mL.
AFP-Specific TCR
We expressed an HLA-A*02:01&B2M&AFP (FMNKFIYEI) TCR which can successfully bind to our single-chain trimer MHC-peptide complex and our prMHC loaded with AFP peptide (FMNKFIYEI).
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Figure 5. Single-chain trimer MHC, Human HLA-A*02:01&B2M&AFP (FMNKFIEl) Monomer, His-Avi Tag captured on CM5 Chip via Anti-His Antibody, can bind HLA-A*02:01&B2M&AFP (FMNKFIYEI) TCR with an affinity constant of 0.3991 µM as determined in SPR assay (Biacore T200).
Figure 6. Peptide-ready MHC, Human HLA-A*02:01&B2M, His-Avi Tag loaded with FMNKFIYEI peptide, captured on CM5 Chip via Anti-His Antibody, can bind HLA-A*02:01&B2M&AFP (FMNKFIYEI) TCR with an affinity constant of 0.326 µM as determined in SPR assay (Biacore T200).
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MHC Monomers & Tetramers
Mammalian-expressed MHC-peptide complexes with verified bioactivity.
Custom MHC Molecule
Custom MHC monomers, tetramers, peptide-ready MHCs, and chimeric MHCs.
Peptide-ready MHCs
Load your own peptide onto a monomer or tetramer in house.
SPR Analysis Service
Surface plasmon resonance services of various sample combinations including antibodies, proteins, peptides, and small molecules