Custom Soluble TCR Expression Service Overview
We're pleased to offer specialized custom services related to MHC and TCR research, including the expression of TCRs, and TCR-related drugs. Researchers often need TCRs in order to study their role in immune responses, to develop TCR-based therapies, and to screen for potential therapeutic candidates. In support of this, we offer custom expression services for soluble TCRs and soluble TCR fusion proteins. Additionally, we can perform analysis services including matured affinity TCR ELISAs and low affinity TCR SPR assays.
TCR Expression & Engineering Service Features

Production of various formats of soluble TCRs, including scFv-TCR, TCR-His, etc.

TCR engineering to optimize soluble TCR expression based on TCR modeling

SPR or ELISA analysis of soluble TCR & MHC interactions
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Case Studies & Applications
GP100 TCR & Anti-CD3 Bispecific Fusion
We have successfully expressed a GP100 TCR & Anti-CD3 Bispecific Fusion, which can not only bind to HLA-A*02:01&B2M&GP100 (YLEPGPVTA) complex, but also to CD3 heterodimers at the same time, demonstrating good bispecific binding activity.

Figure 1. In SPR analysis, the GP100 TCR & Anti-CD3 Bispecific Fusion can bind to the HLA-A*02:01&B2M&GP100 (YLEPGPVTA) complex tetramer, with an affinity constant of 0.196 nM.

Figure 2. ELISA verification demonstrates that GP100 TCR & Anti-CD3 Bispecific Fusion can bind to HLA-A*02:01&B2M&GP100 (YLEPGPVTA) complex monomer with an EC50 of 11.8 ng/mL.

Figure 3. ELISA verification demonstrates that GP100 TCR & Anti-CD3 Bispecific Fusion can bind to Human CD3E&CD3D, with an EC50 value of 8.7 ng/mL.

Figure 4. ELISA verification demonstrates that GP100 TCR & Anti-CD3 Bispecific Fusion can bind to Human CD3E&CD3G, with an EC50 value of 0.22 μg/mL.
AFP-Specific TCR
We expressed an HLA-A*02:01&B2M&AFP (FMNKFIYEI) TCR which can successfully bind to our single-chain trimer MHC-peptide complex and our prMHC loaded with AFP peptide (FMNKFIYEI).

Figure 5. Single-chain trimer MHC, Human HLA-A*02:01&B2M&AFP (FMNKFIEl) Monomer, His-Avi Tag captured on CM5 Chip via Anti-His Antibody, can bind HLA-A*02:01&B2M&AFP (FMNKFIYEI) TCR with an affinity constant of 0.3991 µM as determined in SPR assay (Biacore T200).
Figure 6. Peptide-ready MHC, Human HLA-A*02:01&B2M, His-Avi Tag loaded with FMNKFIYEI peptide, captured on CM5 Chip via Anti-His Antibody, can bind HLA-A*02:01&B2M&AFP (FMNKFIYEI) TCR with an affinity constant of 0.326 µM as determined in SPR assay (Biacore T200).
Why Choose KACTUS for TCR Services?
Production of Various TCR Types
Soluble TCRs, including scFv-TCR and TCR-His; TCR fusion proteins; TCR-CD3 complexes
TCR Engineering
TCR engineering to optimize soluble TCR expression based on TCR modeling
Affinity Analysis
SPR or ELISA analysis of soluble TCR & MHC interactions
Expertise in TCR-MHC interactions
Proven record of expressing TCRs for functional assays and downstream applications