Browse CD3 Proteins
Browse our popular CD3 proteins here or click below to view the full catalog selection:
CD3 Proteins
Highly Active CD3 Proteins for Advanced Immunization and Antibody Research Monomers, homodimers, heterodimers, and biotinylated proteins
Product Features
High Protein Affinity
We verified the activity of our heterodimers, such as CD3E/G-His Tag and CD3E/D-His Tag, via ELISA and SPR.
Equal Expression of Subunits
A proprietary design, expression, and purification system ensures the two subunits of the heterodimers are expressed in equal proportions with greater than 95% purity, verfied by SDS-PAGE and SEC-HPLC.
Stability
Our CD3 proteins have high batch-to-batch consistency and long-term stability as verified by ELISA.
CD3 Proteins Overview
About CD3 Proteins
CD3 (Cluster of differentiation 3) is one of the proteins expressed on the surface of T cells. It consists of six peptide chains, one γ chain, one δ chain, two ε chains and two ζ chains. These six peptide chains are all transmembrane, and the transmembrane region is connected with TCR through a salt bridge to form a tight TCR-CD3 complex, which jointly participates in the recognition and signal transduction of T cells to antigens. The activation signal generated by TCR recognition of antigen is transduced into T cells by the ITAM sequence in the cytoplasmic region of CD3, and then a series of immune responses are initiated.
Applications
Antibody Discovery
Immunization
Antibody Screening
Functional characterization
Affinity Determination (ELISA, SPR, BLI)
TCR/CD3 signaling (Louis-Dit-Sully C et. al, 2012）
Custom CD3 Protein Production
KACTUS offers custom recombinant CD3 protein production.
Product Performance Validation
High Binding Affinity by SPR
Human CD3E/CD3D His tag, captured on CM5 chip via anti-His antibody, binds OKT3 mFc Tag with an affinity constant of 0.36nM as determined in SPR assay (Biacore T200).
Batch-to-Batch Consistency
Immobilized Human CD3E/CD3G, hFc Tag, at 1μg/mL (100μL/well). Dose-response curve for anti-CD3E/CD3G antibody (OKT3, mFc Tag). The EC50s are 20.7, 21.0, and 20.0ng/mL respectively, as determined by ELISA.
Temperature Stability
Immobilized Human CD3E/CD3G, hFc Tag, at 1μg/mL (100μl/well). Dose-response curve for anti-CD3E/CD3G antibody (OKT3, mFc Tag). The EC50s are 20.7, 19.5, 20.0, and 20.7ng/mL respectively, as determined by ELISA.
High Purity by SEC-HPLC
The purity of Cynomolgus CD3E is greater than 95% as determined by SEC-HPLC.
High Purity by Tris-Bis PAGE
Cynomolgus CD3E/CD3D on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (CD3-HM505B)
Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (CD3-HM505B)
from $600.00
Biotinylated Human CD3E/CD3 epsilon 1-27 peptide Protein (CD3-HM2EDB)
Biotinylated Human CD3E/CD3 epsilon 1-27 peptide Protein (CD3-HM2EDB)
from $600.00
Biotinylated Human CD3E/CD3 epsilon Protein (Primary Amine Labeling) (CDE-HM101B)
Biotinylated Human CD3E/CD3 epsilon Protein (Primary Amine Labeling) (CDE-HM101B)
from $525.00
Biotinylated Cynomolgus CD3E/CD3 epsilon Protein (Primary Amine Labeling) (CDE-CM101B)
Biotinylated Cynomolgus CD3E/CD3 epsilon Protein (Primary Amine Labeling) (CDE-CM101B)
from $525.00
Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (Primary Amine Labeling) (CD3-HM105B)
Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (Primary Amine Labeling) (CD3-HM105B)
from $600.00
Biotinylated Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (Primary Amine Labeling) (CD3-HM157B)
Biotinylated Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (Primary Amine Labeling) (CD3-HM157B)
from $600.00
Biotinylated Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (CD3-HM557B)
Biotinylated Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (CD3-HM557B)
from $600.00
Biotinylated Cynomolgus CD3E/CD3 epsilon 1-27 peptide Protein (CD3-CM2EDB)
Biotinylated Cynomolgus CD3E/CD3 epsilon 1-27 peptide Protein (CD3-CM2EDB)
from $600.00
Biotinylated Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (Primary Amine Labeling) (CD3-CM202B)
Biotinylated Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (Primary Amine Labeling) (CD3-CM202B)
from $600.00
Biotinylated Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein (Primary Amine Labeling) (CD3-CM201B)
Biotinylated Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein (Primary Amine Labeling) (CD3-CM201B)
from $525.00
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