CD3 Proteins

Highly Active CD3 Proteins for Advanced Immunization and Antibody Research Monomers, homodimers, heterodimers, and biotinylated proteins

Product Features

High Protein Affinity

We verified the activity of our heterodimers, such as CD3E/G-His Tag and CD3E/D-His Tag, via ELISA and SPR.

Equal Expression of Subunits

A proprietary design, expression, and purification system ensures the two subunits of the heterodimers are expressed in equal proportions with greater than 95% purity, verfied by SDS-PAGE and SEC-HPLC.

Stability

Our CD3 proteins have high batch-to-batch consistency and long-term stability as verified by ELISA.

CD3 Proteins Overview

About CD3 Proteins

CD3 (Cluster of differentiation 3) is one of the proteins expressed on the surface of T cells. It consists of six peptide chains, one γ chain, one δ chain, two ε chains and two ζ chains. These six peptide chains are all transmembrane, and the transmembrane region is connected with TCR through a salt bridge to form a tight TCR-CD3 complex, which jointly participates in the recognition and signal transduction of T cells to antigens. The activation signal generated by TCR recognition of antigen is transduced into T cells by the ITAM sequence in the cytoplasmic region of CD3, and then a series of immune responses are initiated.

Applications

Antibody Discovery

Immunization

Antibody Screening

Functional characterization

Affinity Determination (ELISA, SPR, BLI)

TCR/CD3 signaling (Louis-Dit-Sully C et. al, 2012

Custom CD3 Protein Production

KACTUS offers custom recombinant CD3 protein production.

Product Performance Validation

High Binding Affinity by SPR

Human CD3E/CD3D His tag, captured on CM5 chip via anti-His antibody, binds OKT3 mFc Tag with an affinity constant of 0.36nM as determined in SPR assay (Biacore T200).

Batch-to-Batch Consistency

Immobilized Human CD3E/CD3G, hFc Tag, at 1μg/mL (100μL/well). Dose-response curve for anti-CD3E/CD3G antibody (OKT3, mFc Tag). The EC50s are 20.7, 21.0, and 20.0ng/mL respectively, as determined by ELISA.

Temperature Stability

Immobilized Human CD3E/CD3G, hFc Tag, at 1μg/mL (100μl/well). Dose-response curve for anti-CD3E/CD3G antibody (OKT3, mFc Tag). The EC50s are 20.7, 19.5, 20.0, and 20.7ng/mL respectively, as determined by ELISA.

High Purity by SEC-HPLC

The purity of Cynomolgus CD3E is greater than 95% as determined by SEC-HPLC.

High Purity by Tris-Bis PAGE

Cynomolgus CD3E/CD3D on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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