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Highly Active CD3 Proteins for Advanced Immunization and Antibody Research Monomers, homodimers, heterodimers, and biotinylated proteins
High Protein Affinity
We verified the activity of our heterodimers, such as CD3E/G-His Tag and CD3E/D-His Tag, via ELISA and SPR.
Equal Expression of Subunits
A proprietary design, expression, and purification system ensures the two subunits of the heterodimers are expressed in equal proportions with greater than 95% purity, verfied by SDS-PAGE and SEC-HPLC.
CD3 Proteins Overview
About CD3 Proteins
CD3 (Cluster of differentiation 3) is one of the proteins expressed on the surface of T cells. It consists of six peptide chains, one γ chain, one δ chain, two ε chains and two ζ chains. These six peptide chains are all transmembrane, and the transmembrane region is connected with TCR through a salt bridge to form a tight TCR-CD3 complex, which jointly participates in the recognition and signal transduction of T cells to antigens. The activation signal generated by TCR recognition of antigen is transduced into T cells by the ITAM sequence in the cytoplasmic region of CD3, and then a series of immune responses are initiated.
Affinity Determination (ELISA, SPR, BLI)
TCR/CD3 signaling (Louis-Dit-Sully C et. al, 2012）
Custom CD3 Protein Production
KACTUS offers custom recombinant CD3 protein production.
Product Performance Validation
High Binding Affinity by SPR
Human CD3E/CD3D His tag, captured on CM5 chip via anti-His antibody, binds OKT3 mFc Tag with an affinity constant of 0.36nM as determined in SPR assay (Biacore T200).
Immobilized Human CD3E/CD3G, hFc Tag, at 1μg/mL (100μL/well). Dose-response curve for anti-CD3E/CD3G antibody (OKT3, mFc Tag). The EC50s are 20.7, 21.0, and 20.0ng/mL respectively, as determined by ELISA.
Immobilized Human CD3E/CD3G, hFc Tag, at 1μg/mL (100μl/well). Dose-response curve for anti-CD3E/CD3G antibody (OKT3, mFc Tag). The EC50s are 20.7, 19.5, 20.0, and 20.7ng/mL respectively, as determined by ELISA.
High Purity by SEC-HPLC
The purity of Cynomolgus CD3E is greater than 95% as determined by SEC-HPLC.
High Purity by Tris-Bis PAGE
Cynomolgus CD3E/CD3D on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.