High Protein Affinity
We verified the activity of our heterodimers, such as CD3E/G-His Tag and CD3E/D-His Tag, via ELISA and SPR.
Equal Expression of Subunits
We adopted a specialized design that ensures the two subunits of the heterodimers are expressed in equal proportions with greater than 95% purity.
We demonstrated via ELISA high consistency across batches and long-term stability of our products.
Affinity Determination (ELISA, SPR, BLI)
Figure 1. Immobilized Human CD3E/CD3G, hFc Tag, at 1μg/mL (100μl/well). Dose-response curve for anti-CD3E/CD3G antibody (OKT3, mFc Tag). The EC50s are 20.7, 19.5, 20.0, and 20.7ng/mL respectively, as determined by ELISA.
Figure 2. Human CD3E/CD3D His tag, captured on CM5 chip via anti-His antibody, binds OKT3 mFc Tag with an affinity constant of 0.36nM as determined in SPR assay (Biacore T200).
Figure 3. Immobilized Human CD3E/CD3G, hFc Tag, at 1μg/mL (100μL/well). Dose-response curve for anti-CD3E/CD3G antibody (OKT3, mFc Tag). The EC50s are 20.7, 21.0, and 20.0ng/mL respectively, as determined by ELISA.
Figure 4.Human CD3E/CD3G His tag, captured on CM5 Chip via anti-His antibody, can bind OKT mFc tag with an affinity constant of 0.22nM as determined in SPR assay (Biacore T200).
Figure 5.The purity of Cynomolgus CD3E is greater than 95% as determined by SEC-HPLC.
Figure 6.Cynomolgus CD3E/CD3D on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.