ELISA binding curve showing dose-dependent interaction between anti-GPC3 antibody and mouse GPC3 His-tag protein, measured by OD450 absorbance.
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse GPC3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Gln25-Met557
  • Other Available Proteins:
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Catalog #GPC-MM131
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Mouse GPC3/Glypican 3 Protein (GPC-MM131) Summary

Product Description

 Recombinant Mouse GPC3/Glypican 3 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gln25-Met557.

Product Background

 Glypican-3 is a protein ,which is encoded by the GPC3 gene in humans.The protein core of GPC3 consists of two subunits, where the N-terminal subunit has a size of ~40 kDa and the C-terminal subunit is ~30 kDa.Glypican 3 is a potential therapeutic target for treating liver cancer and other cancers. Several therapeutic anti-GPC3 antibodies have been developed.

Product Category

 Recombinant Protein / Antibody discovery, Cell therapy / ADC targets

Mouse GPC3/Glypican 3 Protein (GPC-MM131) Specifications/Details

Protein
 GPC3/Glypican 3
Synonyms
 GPC3; DGSX; Glypican 3; GTR2-2; MXR7; OCI5; OCI-5; SGB; SGBS; SGBS1SDYS; SDYS; SGBS1
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Gln25-Met557
Molecular Weight
 The protein has a predicted MW of 61.62 kDa. Due to furin-like convertase cleavage site and glycosylation, the protein migrates to 32-42 kDa and 75-85 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Mouse GPC3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA (QC Test).
Product Tag
 C-His

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Mouse GPC3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA (QC Test). Immobilized Mouse GPC3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA (QC Test).
Mouse GPC3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse GPC3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse GPC3 is greater than 95% as determined by SEC-HPLC. The purity of Mouse GPC3 is greater than 95% as determined by SEC-HPLC.
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