ELISA binding curve showing dose-dependent interaction between anti-GPC3 antibody and mouse GPC3 His-tag protein, measured by OD450 absorbance.
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 90% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse GPC3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Gln25-Met557
  • Other Available Proteins:
    Finding available proteins...
Catalog #GPC-MM131
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Size
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Mouse GPC3/Glypican 3 Protein (GPC-MM131) Summary

Product Description

 Recombinant Mouse GPC3/Glypican 3 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gln25-Met557.

Product Background

 Glypican-3 is a protein ,which is encoded by the GPC3 gene in humans.The protein core of GPC3 consists of two subunits, where the N-terminal subunit has a size of ~40 kDa and the C-terminal subunit is ~30 kDa.Glypican 3 is a potential therapeutic target for treating liver cancer and other cancers. Several therapeutic anti-GPC3 antibodies have been developed.

Product Category

 Recombinant Protein / Antibody discovery, Cell therapy / ADC targets

Mouse GPC3/Glypican 3 Protein (GPC-MM131) Specifications/Details

Protein
 GPC3/Glypican 3
Synonyms
 GPC3; DGSX; Glypican 3; GTR2-2; MXR7; OCI5; OCI-5; SGB; SGBS; SGBS1SDYS; SDYS; SGBS1
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Gln25-Met557
Molecular Weight
 The protein has a predicted MW of 61.62 kDa. Due to furin-like convertase cleavage site and glycosylation, the protein migrates to 32-42 kDa and 75-85 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Mouse GPC3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA (QC Test).
Product Tag
 C-His

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 90% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Mouse GPC3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA (QC Test). Immobilized Mouse GPC3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA (QC Test).
Mouse GPC3 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%. Mouse GPC3 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
The purity of Mouse GPC3 is greater than 95% as determined by SEC-HPLC. The purity of Mouse GPC3 is greater than 95% as determined by SEC-HPLC.
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