Mouse GPC3/Glypican 3 Protein (GPC-MM131)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Mouse GPC3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Gln25-Met557

Catalog: GPC-MM131

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Product Description	Recombinant Mouse GPC3/Glypican 3 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gln25-Met557.
Product Background	Glypican-3 is a protein ,which is encoded by the GPC3 gene in humans.The protein core of GPC3 consists of two subunits, where the N-terminal subunit has a size of ~40 kDa and the C-terminal subunit is ~30 kDa.Glypican 3 is a potential therapeutic target for treating liver cancer and other cancers. Several therapeutic anti-GPC3 antibodies have been developed.
Product Category	Recombinant Protein

Target	GPC3/Glypican 3
Gene/Protein ID	Q8CFZ4
Species	Mouse
Exact Sequence	Gln25-Met557
Molecular Weight	The protein has a predicted MW of 61.62 kDa. Due to furin-like convertase cleavage site and glycosylation, the protein migrates to 32-42 kDa and 75-85 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Mouse GPC3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Mouse GPC3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA.
	The purity of Mouse GPC3 is greater than 95% as determined by SEC-HPLC.
	Mouse GPC3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.