PE-Labeled Human GPC3/Glypican 3 Protein (GPC-HM131P)
Catalog: GPC-HM131P
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Product Description | Recombinant PE-Labeled Human GPC3/Glypican 3 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gln25-His559. |
Product Background |
Glypican-3 is a protein ,which is encoded by the GPC3 gene in humans.The protein core of GPC3 consists of two subunits, where the N-terminal subunit has a size of ~40 kDa and the C-terminal subunit is ~30 kDa.Glypican 3 is a potential therapeutic target for treating liver cancer and other cancers. Several therapeutic anti-GPC3 antibodies have been developed. |
Product Category |
Recombinant Protein |
Target |
GPC3/Glypican 3 |
Gene/Protein ID |
P51654-1 |
Species |
Human |
Exact Sequence |
Gln25-His559 |
Molecular Weight | The protein has a predicted MW of 61.6 kDa. |
Activity |
Immobilized PE-Labeled Human GPC3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Fluorophore |
PE |
Express System |
HEK293 |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose / 8% mannitol is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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