ELISA assay curve of PE-labeled human GPC3 binding to anti-GPC3 antibody, showing OD450 response versus antibody concentration.
PE-Labeled Human GPC3/Glypican 3 Protein (GPC-HM131P)
  • Express System: HEK293
  • Product Tag: C-His
  • Activity: Immobilized PE-Labeled Human GPC3, His Tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 3.2 ng/ml determined by ELISA.
  • Amino Acid Range: Gln25-His559
Catalog #GPC-HM131P
Regular price $990.00
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PE-Labeled Human GPC3/Glypican 3 Protein (GPC-HM131P) Summary

Product Description

Recombinant PE-Labeled Human GPC3/Glypican 3 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gln25-His559.

Product Background

Glypican-3 is a protein, which is encoded by the GPC3 gene in humans. The protein core of GPC3 consists of two subunits, where the N-terminal subunit has a size of ~40 kDa and the C-terminal subunit is ~30 kDa. Glypican 3 is a potential therapeutic target for treating liver cancer and other cancers. Several therapeutic anti-GPC3 antibodies have been developed.

Product Category

Recombinant Protein / Antibody discovery, Cell therapy / ADC targets

PE-Labeled Human GPC3/Glypican 3 Protein (GPC-HM131P) Specifications/Details

Protein
GPC3/Glypican 3
Synonyms
GPC3; DGSX; Glypican 3; GTR2-2; MXR7; OCI5; OCI-5; SGB; SGBS; SGBS1SDYS; SDYS; SGBS1
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Gln25-His559
Molecular Weight
The protein has a predicted MW of 61.6 kDa.
Activity
Immobilized PE-Labeled Human GPC3, His Tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 3.2 ng/ml determined by ELISA.
Product Tag
C-His

Fluorophore

PE
Expression System
HEK293
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
Valid for 6 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized PE-Labeled Human GPC3, His Tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 3.2 ng/ml determined by ELISA. Immobilized PE-Labeled Human GPC3, His Tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 3.2 ng/ml determined by ELISA.
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