PE-Labeled Human GPC3/Glypican 3 Protein (GPC-HM131P)

  • Express System: HEK293
  • Product Tag: C-His
  • Activity: Immobilized PE-Labeled Human GPC3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln25-His559

Catalog: GPC-HM131P

$900.00
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Product Description Recombinant PE-Labeled Human GPC3/Glypican 3 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gln25-His559.

Product Background

Glypican-3 is a protein ,which is encoded by the GPC3 gene in humans.The protein core of GPC3 consists of two subunits, where the N-terminal subunit has a size of ~40 kDa and the C-terminal subunit is ~30 kDa.Glypican 3 is a potential therapeutic target for treating liver cancer and other cancers. Several therapeutic anti-GPC3 antibodies have been developed.

Product Category

Recombinant Protein

Target

GPC3/Glypican 3

Gene/Protein ID

P51654-1

Species

Human

Exact Sequence

Gln25-His559
Molecular Weight The protein has a predicted MW of 61.6 kDa.

Activity

Immobilized PE-Labeled Human GPC3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Fluorophore

PE

Express System

HEK293
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose / 8% mannitol is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized PE-Labeled Human GPC3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA.

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