PE-Labeled Human GPC3/Glypican 3 Protein (GPC-HM131P)
  • Express System: HEK293
  • Product Tag: C-His
  • Activity: Immobilized PE-Labeled Human GPC3, His Tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 4.4 ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln25-His559
Catalog #GPC-HM131P
Regular price $900.00
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Product Description
Recombinant PE-Labeled Human GPC3/Glypican 3 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gln25-His559.
Product Background
Glypican-3 is a protein, which is encoded by the GPC3 gene in humans. The protein core of GPC3 consists of two subunits, where the N-terminal subunit has a size of ~40 kDa and the C-terminal subunit is ~30 kDa. Glypican 3 is a potential therapeutic target for treating liver cancer and other cancers. Several therapeutic anti-GPC3 antibodies have been developed.
Product Category
Recombinant Protein
Target
GPC3/Glypican 3
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Gln25-His559
Molecular Weight
The protein has a predicted MW of 61.6 kDa.
Activity
Immobilized PE-Labeled Human GPC3, His Tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 4.4 ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His
Fluorophore
PE
Express System
HEK293
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose / 8% mannitol is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized PE-Labeled Human GPC3, His Tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 4.4 ng/ml determined by ELISA.

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