MHC Tetramer Staining for Flow Cytometry in Whole Blood, PBMCs, and Spleen Cells | Protocol

Download this step-by-step protocol for performing MHC tetramer staining to detect antigen-specific T cells using flow cytometry. This protocol is optimized for use with both whole blood samples and cell suspensions (e.g., PBMCs, mouse spleen cells), and includes an optional peptide pulsing step to expand T cells prior to staining.